School of Basic Medical Science, Guangxi University of Chinese Medicine, Nanning 530200, China.
Endocr Metab Immune Disord Drug Targets. 2023;23(7):927-936. doi: 10.2174/1871530323666230206160722.
Modern lifestyle increasingly deprives people from sleep to different degrees. Long-term sleep deprivation will facilitate body's pathological behaviors, such as lethargy, depression, and anorexia.
This study is an investigation into the mechanism of hydrolyzed seawater pearl tablet in treating chronic sleep deprivation mice model.
The chronic sleep deprivation model was established involving C57BL/6mice; the body weight, behavioral characteristics, hippocampal structure, oxidative stress, apoptosis-related protein expression, and intestinal bacteria in mice were assessed to characterise hydrolyzed seawater pearl tablet.
Hydrolyzed seawater pearl tablet significantly accelerated body weight, open field test score, and sugar water preference rate (P < 0.05), alleviated the structural damage of hippocampus, reduced the content of MDA (P < 0.05), Bax protein expression, increased the content of GSH (P < 0.05), the activities of SOD, GSH-Px, and Bcl-2 protein expression in the hippocampus, increased the Escherichia coli, Bacteroides, Bifidobacterium and Lactobacillus (P < 0.05), which are beneficial bacteria in the intestine, in chronic sleep deprivation mice, and reduced the amount of Clostridium perfringens (P < 0.05), which are harmful bacteria in the intestine.
Hydrolyzed seawater pearl tablet can improve the depression-like mental state of mice caused by chronic sleep deprivation. The mechanism involves improving the antioxidant activity of the hippocampus to eliminate the excessive ROS, which inhibits cell apoptosis and alleviates tissue structure damage. Meanwhile, it may also be involved in adjusting the microbiota level and improving the mental and behavioral activities of chronic sleep deprivation mice through the intestine-brain axis.
现代生活方式日益剥夺人们的睡眠时间，程度不同。长期睡眠剥夺会促进身体的病理性行为，如嗜睡、抑郁和厌食。
本研究旨在探讨水解海水珍珠片治疗慢性睡眠剥夺小鼠模型的机制。
采用 C57BL/6mice 建立慢性睡眠剥夺模型；评估小鼠的体重、行为特征、海马结构、氧化应激、凋亡相关蛋白表达和肠道细菌，以表征水解海水珍珠片。
水解海水珍珠片显著加速了小鼠的体重、旷场试验得分和糖水偏好率（P < 0.05），缓解了海马体的结构损伤，降低了 MDA（P < 0.05）、Bax 蛋白表达的含量，增加了 GSH（P < 0.05）的含量、海马体中 SOD、GSH-Px 和 Bcl-2 蛋白表达的活性，增加了大肠杆菌、拟杆菌、双歧杆菌和乳酸杆菌（P < 0.05）等有益肠道细菌的数量，减少了梭状芽孢杆菌（P < 0.05）等有害肠道细菌的数量。
水解海水珍珠片可改善慢性睡眠剥夺引起的小鼠抑郁样精神状态。其机制涉及改善海马体的抗氧化活性以消除过多的 ROS，抑制细胞凋亡并减轻组织结构损伤。同时，它可能还通过肠-脑轴通过调节微生物群水平和改善慢性睡眠剥夺小鼠的精神和行为活动来发挥作用。
