通过与玻璃珠搅拌进行酵母转化。
Transformation of yeast by agitation with glass beads.
作者信息
Costanzo M C, Fox T D
机构信息
Section of Genetics and Development, Cornell University, Ithaca, New York 14853.
出版信息
Genetics. 1988 Nov;120(3):667-70. doi: 10.1093/genetics/120.3.667.
Abstract
We have found that agitation of Saccharomyces cerevisiae with glass beads and plasmid DNA using a vortex mixer results in genetic transformation of the yeast cells. This method is less efficient, but considerably more convenient, than other yeast transformation procedures. The fact that the minimal requirements for transformation are simply physical damage and the presence of DNA in an osmotically supportive environment suggests that this process may occur in nature.
摘要
我们发现，使用涡旋混合器将酿酒酵母与玻璃珠和质粒DNA一起搅拌可导致酵母细胞的遗传转化。与其他酵母转化程序相比，该方法效率较低，但更为便捷。转化的最低要求仅仅是物理损伤以及在渗透支持环境中存在DNA，这一事实表明该过程可能在自然中发生。
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