Pochini Lorena, Galluccio Michele
Laboratory of Biochemistry, Molecular Biotechnology, and Molecular Biology, Department of Biology, Ecology and Earth Sciences (DiBEST), University of Calabria, Via P. Bucci 4c, 87036 Rende, Italy.
Life (Basel). 2022 Aug 8;12(8):1206. doi: 10.3390/life12081206.
For more than 20 years, yeast has been a widely used system for the expression of human membrane transporters. Among them, more than 400 are members of the largest transporter family, the SLC superfamily. SLCs play critical roles in maintaining cellular homeostasis by transporting nutrients, ions, and waste products. Based on their involvement in drug absorption and in several human diseases, they are considered emerging therapeutic targets. Despite their critical role in human health, a large part of SLCs' is 'orphans' for substrate specificity or function. Moreover, very few data are available concerning their 3D structure. On the basis of the human health benefits of filling these knowledge gaps, an understanding of protein expression in systems that allow functional production of these proteins is essential. Among the 500 known yeast species, and represent those most employed for this purpose. This review aims to provide a comprehensive state-of-the-art on the attempts of human SLC expression performed by exploiting yeast. The collected data will hopefully be useful for guiding new attempts in SLCs expression with the aim to reveal new fundamental data that could lead to potential effects on human health.
20多年来，酵母一直是广泛用于表达人类膜转运蛋白的系统。其中，超过400种是最大的转运蛋白家族——SLC超家族的成员。SLC通过转运营养物质、离子和废物在维持细胞稳态中发挥关键作用。基于它们在药物吸收和多种人类疾病中的作用，它们被视为新兴的治疗靶点。尽管它们在人类健康中起着关键作用，但很大一部分SLC在底物特异性或功能方面仍是“孤儿”。此外，关于它们三维结构的数据非常少。基于填补这些知识空白对人类健康的益处，了解能够功能性生产这些蛋白质的系统中的蛋白质表达至关重要。在500种已知的酵母物种中，[此处可能有缺失信息]和[此处可能有缺失信息]是最常用于此目的的物种。本综述旨在全面介绍利用酵母进行人类SLC表达尝试的最新进展。收集到的数据有望有助于指导SLC表达的新尝试，以揭示可能对人类健康产生潜在影响的新基础数据。
