Institute of Natural Sciences, Massey University, Albany, New Zealand.
PLoS One. 2012;7(1):e30943. doi: 10.1371/journal.pone.0030943. Epub 2012 Jan 23.
Nuclear and mitochondrial organelles must maintain a communication system. Loci on the mitochondrial genome were recently reported to interact with nuclear loci. To determine whether this is part of a DNA based communication system we used genome conformation capture to map the global network of DNA-DNA interactions between the mitochondrial and nuclear genomes (Mito-nDNA) in Saccharomyces cerevisiae cells grown under three different metabolic conditions. The interactions that form between mitochondrial and nuclear loci are dependent on the metabolic state of the yeast. Moreover, the frequency of specific mitochondrial-nuclear interactions (i.e. COX1-MSY1 and Q0182-RSM7) showed significant reductions in the absence of mitochondrial encoded reverse transcriptase machinery. Furthermore, these reductions correlated with increases in the transcript levels of the nuclear loci (MSY1 and RSM7). We propose that these interactions represent an inter-organelle DNA mediated communication system and that reverse transcription of mitochondrial RNA plays a role in this process.
核和线粒体细胞器必须维持一个通讯系统。最近有报道称，线粒体基因组上的基因座与核基因座相互作用。为了确定这是否是基于 DNA 的通讯系统的一部分，我们使用基因组构象捕获技术来绘制酿酒酵母细胞在三种不同代谢条件下线粒体和核基因组（Mito-nDNA）之间的 DNA-DNA 相互作用的全局网络。线粒体和核基因座之间形成的相互作用取决于酵母的代谢状态。此外，在缺乏线粒体编码的逆转录酶机制的情况下，特定的线粒体-核相互作用（即 COX1-MSY1 和 Q0182-RSM7）的频率显著降低。此外，这些减少与核基因座（MSY1 和 RSM7）的转录本水平的增加相关。我们提出这些相互作用代表一种细胞器间的 DNA 介导通讯系统，并且线粒体 RNA 的逆转录在该过程中起作用。
