Center for Industrial Diagnostics and Fluid Dynamics (CDIF), Polytechnic University of Catalonia (UPC), 08034 Barcelona, Spain.
Experimental Trauma Surgery, Justus Liebig University of Giessen, Germany.
Sensors (Basel). 2019 Jan 21;19(2):436. doi: 10.3390/s19020436.
In this present study, we propose a method for exposing biological cells to mechanical vibration. The motive for our research was to design a bioreactor prototype in which in-depth in vitro studies about the influence of vibration on cells and their metabolism can be performed. The therapy of cancer or antibacterial measures are applications of interest. In addition, questions about the reaction of neurons to vibration are still largely unanswered. In our methodology, we used a piezoelectric patch (PZTp) for inducing mechanical vibration to the structure. To control the vibration amplitude, the structure could be excited at different frequency ranges, including resonance and non-resonance conditions. Experimental results show the vibration amplitudes expected for every frequency range tested, as well as the vibration pattern of those excitations. These are essential parameters to quantify the effect of vibration on cell behavior. Furthermore, a numerical model was validated with the experimental results presenting accurate results for the prediction of those parameters. With the calibrated numerical model, we will study in greater depth the effects of different vibration patterns for the abovementioned cell types.
在本研究中，我们提出了一种使生物细胞暴露于机械振动的方法。我们研究的动机是设计一种生物反应器原型，在其中可以进行关于振动对细胞及其代谢影响的深入体外研究。癌症治疗或抗菌措施是感兴趣的应用。此外，关于神经元对振动的反应的问题在很大程度上仍未得到解答。在我们的方法中，我们使用压电贴片（PZTp）来对结构施加机械振动。为了控制振动幅度，可以在不同的频率范围内激励结构，包括共振和非共振条件。实验结果显示了每个测试频率范围内预期的振动幅度以及这些激励的振动模式。这些是量化振动对细胞行为影响的重要参数。此外，数值模型通过实验结果进行了验证，这些结果为这些参数的预测提供了准确的结果。利用经过校准的数值模型，我们将更深入地研究不同振动模式对上述细胞类型的影响。
