Jiang Yanni, Zhao Yi, Liu Yi, Huang Qiaorong, Meng Wentong, Xu Hong, Mo Xianming
Department of Rheumatology and Immunology, West China Hospital, Sichuan University, Chengdu, China.
Laboratory of Stem Cell Biology, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University and Collaborative Innovation Center For Biotherapy, Chengdu, China.
Arch Rheumatol. 2020 Jun 2;35(4):521-532. doi: 10.46497/ArchRheumatol.2020.7440. eCollection 2020 Dec.
This study aims to evaluate the frequency and absolute number of circulating innate lymphoid cell (ILC) subsets and their associations with clinical and serological features in systemic lupus erythematosus (SLE).
We recruited 28 SLE patients (6 males, 22 females; mean age 37.57 years; range, 18 to 56 years) and 13 healthy controls (4 males, 9 females; mean age 32.08 years; range, 19 to 48 years). Circulating ILC subsets were identified by flow cytometry. Associations between all detected cells and SLE disease activity, clinical manifestations, and serum autoantibodies were analyzed.
In this study, significantly higher frequencies of ILC2s and ILC3s, lower frequencies of ILC1s, and higher ILC1/ILC3 and ILC1/ILC2 ratios were observed in SLE patients than in healthy controls. The frequencies and number of ILC3s were positively associated with SLE disease activity index 2000 score and anti-double stranded deoxyribonucleic acid titers in patients with SLE. Decreased ILC1 frequencies, increased ILC3 frequencies, and decreased ILC1/ILC3 and ILC2/ILC3 ratios were observed in patients with arthritis compared to those without arthritis.
Our results indicated biased altered distributions of circulating ILC subsets in SLE. ILC3s were associated with SLE disease activity, and ILC1s, ILC3s, and ILC1/ILC3 and ILC2/ILC3 ratios were associated with SLE accompanied with arthritis. Taken together, these results suggest that ILCs may serve as cellular biomarkers for disease activity and arthritis involvement in SLE.
本研究旨在评估系统性红斑狼疮（SLE）患者循环中固有淋巴细胞（ILC）亚群的频率和绝对数量，及其与临床和血清学特征的相关性。
我们招募了28例SLE患者（6例男性，22例女性；平均年龄37.57岁；范围18至56岁）和13名健康对照者（4例男性，9例女性；平均年龄32.08岁；范围19至48岁）。通过流式细胞术鉴定循环中的ILC亚群。分析所有检测细胞与SLE疾病活动度、临床表现和血清自身抗体之间的相关性。
在本研究中，与健康对照者相比，SLE患者中ILC2和ILC3的频率显著更高，ILC1的频率更低，且ILC1/ILC3和ILC1/ILC2比值更高。SLE患者中ILC3的频率和数量与SLE疾病活动指数2000评分及抗双链脱氧核糖核酸滴度呈正相关。与无关节炎的患者相比，关节炎患者的ILC1频率降低，ILC3频率升高，ILC1/ILC3和ILC2/ILC3比值降低。
我们的结果表明SLE患者循环中ILC亚群的分布存在偏向性改变。ILC3与SLE疾病活动相关，ILC1、ILC3以及ILC1/ILC3和ILC2/ILC3比值与伴有关节炎的SLE相关。综上所述，这些结果提示ILC可能作为SLE疾病活动和关节炎累及的细胞生物标志物。
