Chun Ju Lan, Bang Han Tae, Ji Sang Yun, Jeong Jin Young, Kim Minji, Kim Byeonghyeon, Lee Sung Dae, Lee Yoo Kyung, Reddy Kondreddy Eswar, Kim Ki Hyun
National Institute of Animal Science, Rural Development Administration, Wanju 55365, Korea.
J Anim Sci Technol. 2020 Jan;62(1):58-63. doi: 10.5187/jast.2020.62.1.58. Epub 2020 Jan 31.
The pre-treatment condition affects on the element analysis of inductively coupled plasma (ICP). In this study the pre-treatment condition of ICP has been studied to quantify elements in dog's hair. The hair samples were collected from twelve female Beagles by clipping them into 1 or 2 cm at the back neck. The qualitative and quantitative analysis of elements in hairs were performed by using ICP. By ICP nine elements were qualitatively detected and quantitatively analyzed (Ca, Cu, Fe, K, Mg, Na, P, Se, Zn). The measured amounts of elements were compared between 3 step and 2 step procedures which were with and without the acetone based washing step. The quantitative analysis showed that the concentrations of K, Na, P, and Se were significantly decreased in hair samples with acetone-based washing ( < 0.005 or 0.001) unlike those without the acetone-based washing. It implied that some minerals are lost by the acetone based washing during the sample preparation step. Therefore, the acetone based washing process is not suitable for quantifying elements in dog's hair. In addition, the results of qualitative and quantitative analysis were compared. Although there was a difference in absolute values of elemental contents in hair, the results of qualitative and quantitative analysis were significantly correlated each other. This finding suggested that the results of qualitative analysis can be used to monitor elemental contents in dog's hair.
预处理条件会影响电感耦合等离子体（ICP）的元素分析。在本研究中，对ICP的预处理条件进行了研究，以定量分析犬毛中的元素。从12只雌性比格犬的后颈部采集毛发样本，将其剪成1或2厘米长。使用ICP对毛发中的元素进行定性和定量分析。通过ICP定性检测并定量分析了9种元素（钙、铜、铁、钾、镁、钠、磷、硒、锌）。比较了三步法和两步法（有无基于丙酮的洗涤步骤）中元素的测量量。定量分析表明，与无基于丙酮洗涤的毛发样本相比，有基于丙酮洗涤的毛发样本中钾、钠、磷和硒的浓度显著降低（<0.005或0.001）。这意味着在样品制备步骤中，基于丙酮的洗涤会导致一些矿物质流失。因此，基于丙酮的洗涤过程不适用于定量分析犬毛中的元素。此外，还比较了定性和定量分析的结果。尽管毛发中元素含量的绝对值存在差异，但定性和定量分析的结果彼此显著相关。这一发现表明，定性分析的结果可用于监测犬毛中的元素含量。
