Central Laboratory, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
Department of Chemistry, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
Biomed Res Int. 2018 Dec 23;2018:6010572. doi: 10.1155/2018/6010572. eCollection 2018.
Increasing reports of infectious diseases worldwide have become a global concern in recent times. Depleted antibiotic pipelines, rapid and complex cases of antimicrobial resistance, and emergence and re-emergence of infectious disease have necessitated an urgent need for the development of new antimicrobial therapeutics, preferably with novel modes of action. Due to their distinct mode of action, antimicrobial peptides offer an interesting alternative to conventional antibiotics to deal with the problems enumerated. In this study, the antimicrobial potential of the peptide extract from the marine mollusc, was evaluated Agar diffusion and broth dilution techniques were used to evaluate microbial susceptibility to the peptide extract. Microplate-based assays were also used to investigate time-dependent growth inhibition profiles of microbes in the presence of peptide and evaluate the peptide's ability to modulate the activities of standard antibiotics. Both Gram-positive and Gram-negative bacteria were inhibited by the peptide extract in the agar diffusion assay. The minimum inhibitory concentration (MIC) of peptide against test microorganisms was between 0.039 and 2.5 mg/mL. At the MIC, the peptide extract was bacteriostatic towards all tested microorganisms but bactericidal to In the presence of the peptide extract, a prolonged lag phase was observed for all microbes, similar to standard ciprofloxacin. When administered together, peptide extracts enhanced the activities of ciprofloxacin and cefotaxime and were antagonistic towards erythromycin but indifferent towards metronidazole. Taken together, these results show the broad-spectrum antibacterial activity of peptide extract from and demonstrate that antimicrobial peptides can be employed in combination with some conventional antibiotics for improved effects.
近年来，全球不断增加的传染病报告已成为全球关注的焦点。抗生素储备减少、快速而复杂的抗菌药物耐药性问题以及传染病的出现和再次出现，都迫切需要开发新的抗菌治疗药物，最好具有新颖的作用模式。由于其独特的作用模式，抗菌肽为解决上述问题提供了一种有吸引力的替代传统抗生素的方法。在本研究中，评估了来自海洋软体动物的肽提取物的抗菌潜力。采用琼脂扩散和肉汤稀释技术评估了微生物对肽提取物的敏感性。还使用微孔板测定法研究了在存在肽的情况下微生物的时间依赖性生长抑制曲线，并评估了肽调节标准抗生素活性的能力。琼脂扩散试验中，肽提取物对革兰氏阳性菌和革兰氏阴性菌均有抑制作用。肽对测试微生物的最小抑菌浓度（MIC）在 0.039 至 2.5mg/mL 之间。在 MIC 下，肽提取物对所有测试微生物均具有抑菌作用，但对 具有杀菌作用。在肽提取物存在的情况下，所有微生物的迟滞期均延长，与标准环丙沙星相似。当一起给药时，肽提取物增强了环丙沙星和头孢噻肟的活性，对红霉素具有拮抗作用，但对甲硝唑无影响。综上所述，这些结果表明 肽提取物具有广谱的抗菌活性，并证明抗菌肽可以与某些传统抗生素联合使用以提高效果。
