中国太行山野生猕猴（猕猴华北亚种）中内阿米巴属的分子特征分析
Molecular Characterization of Entamoeba spp. in Wild Taihangshan Macaques (Macaca mulatta tcheliensis) in China.
作者信息
Zhang Qingxun, Liu Kongshang, Wang Chengmin, Luo Jing, Lu Jiqi, He Hongxuan
机构信息
National Research Center for Wildlife-Borne Diseases, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
University of the Chinese Academy of Sciences, Beijing, China.
出版信息
Acta Parasitol. 2019 Jun;64(2):228-231. doi: 10.2478/s11686-019-00026-y. Epub 2019 Jan 22.
BACKGROUND
Entamoeba spp. is one of the most common enteric parasites of humans and animals.
PURPOSE
However, little information is available regarding prevalence and genotypes of Entamoeba spp. in wild Taihangshan macaques (Macaca mulatta tcheliensis).
METHODS
In the present study, a total of 458 fecal samples from wild rhesus macaque in Taihangshan mountains area between September 2015 and November 2016, were collected and examined for the presence of six Entamoeba species by PCR amplification of the SSU rRNA gene.
RESULTS
The overall prevalence of Entamoeba spp. infection was 89.96% (412/458). Four species of Entamoeba detected in our survey were E. chattoni (88.43%), E. hartmanni (79.91%), E. coil (69.87%) and E. dispar (58.30%), and among these, 398 (84.93%) were mixed infections. Phylogenetic analysis revealed that isolates were clustered into four known genotypes.
CONCLUSION
The present study firstly provides important information about the prevalence and diversity of Entamoeba species infecting wild Taihangshan macaques in China. Enough attention should be paid to monitor the potential interspecies transmission in the region.
背景
内阿米巴属是人和动物中最常见的肠道寄生虫之一。
目的
然而，关于太行山野生猕猴（Macaca mulatta tcheliensis）体内内阿米巴属的流行情况和基因型的信息却很少。
方法
在本研究中，于2015年9月至2016年11月期间，从太行山地区的野生恒河猴采集了总共458份粪便样本，并通过对小亚基核糖体RNA（SSU rRNA）基因进行PCR扩增来检测六种内阿米巴物种的存在情况。
结果
内阿米巴属感染的总体患病率为89.96%（412/458）。在我们的调查中检测到的四种内阿米巴物种分别是查顿内阿米巴（88.43%）、哈氏内阿米巴（79.91%）、结肠内阿米巴（69.87%）和迪斯帕内阿米巴（58.30%），其中398例（84.93%）为混合感染。系统发育分析表明，分离株聚类为四个已知基因型。
结论
本研究首次提供了关于感染中国太行山野生猕猴的内阿米巴物种的流行情况和多样性的重要信息。应充分重视监测该地区潜在的种间传播。
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