Terezinha de Oliveira Cledes, Lameiro de Noronha Sales Maia Beatriz Helena, Portes Ferriani Aurea, Aparecida Queiroz Santos Vidiany, Antônio Alves da Cunha Mário, Dias Teixeira Sirlei
Instituto Federal de Educação, Ciência e Tecnologia do Paraná (IFPR), Av. Bento Munhoz da Rocha Neto, PRT 280, 85555-000, Palmas, PR, Brasil.
Departamento de Química, Universidade Federal do Paraná (UFPR), Jardim das Américas, 81530-990, Curitiba, PR, Brasil.
Chem Biodivers. 2019 Feb;16(2):e1800372. doi: 10.1002/cbdv.201800372. Epub 2019 Jan 23.
This is the first time that composition, antimicrobial potential and antioxidant ability of essential oil from the leaves of Baccharis oreophila are reported. Essential oil was obtained by hydrodistillation and analyzed by GC/MS. Antimicrobial potential was evaluated by diffusion disk and broth microdilution methods. ABTS , DPPH and FRAP methods were employed for antioxidant activity evaluation. Essential oil yield was 0.47 %. Sixty-five compounds were identified, representing 88.53 % of the total essential oil, which showed to be rich in oxygenated (37.88 %) and hydrocarbons sesquiterpenes (34.84 %). The main constituents were khusimone (16.37 %) and spathulenol (16.12 %). Antimicrobial activity was verified against S. aureus (10.33±0.5 mm, MIC: 1250 μg mL ) and C. albicans (8.66±0.5 mm, MIC: >2500 μg mL ). Antioxidant ability was evidenced by FRAP (4.09 μmol FeSO E mL ), ABTS (1.45 μmol TE mL ) and DPPH (1.04 μmol TE mL ) scavenging capacity. Results showed that this essential oil has interesting biological potential, encouraging further investigations especially in relation to action mechanisms of antimicrobial and antioxidant activity.
这是首次报道奥氏酒神菊叶片精油的成分、抗菌潜力和抗氧化能力。通过水蒸馏法获得精油，并采用气相色谱/质谱联用仪进行分析。通过扩散圆盘法和肉汤微量稀释法评估抗菌潜力。采用ABTS法、DPPH法和FRAP法评估抗氧化活性。精油得率为0.47%。鉴定出65种化合物，占精油总量的88.53%，结果表明该精油富含氧化合物（37.88%）和倍半萜烃类化合物（34.84%）。主要成分是阔苞菊酮（16.37%）和匙叶桉油烯醇（16.12%）。验证了该精油对金黄色葡萄球菌（抑菌圈直径为10.33±0.5 mm，最低抑菌浓度：1250 μg mL ）和白色念珠菌（抑菌圈直径为8.66±0. 5mm，最低抑菌浓度：>2500 μg mL ）的抗菌活性。通过FRAP法（4.09 μmol FeSO E mL ）、ABTS法（1.45 μmol TE mL ）和DPPH法（1.04 μmol TE mL ）的清除能力证明了其抗氧化能力。结果表明，这种精油具有有趣的生物学潜力，尤其在抗菌和抗氧化活性作用机制方面，鼓励进一步开展研究。
