Universidad Nacional de Colombia-Palmira, Colombia.
Department of Crop Sciences, College of Agricultural and Marine Sciences, Sultan Qaboos University, Al-Khod 123, Oman.
Plant Dis. 2018 Jan;102(1):49-59. doi: 10.1094/PDIS-01-17-0068-RE. Epub 2017 Nov 17.
Common guava (Psidium guajava L.) is a fruit tree of global economic importance. It is grown in Asia, South and Central America, and Hawaii for its exquisite aroma and flavor, and nutritional and medical properties. However, guava production is limited by guava scab, caused by fungi in the Pestalotiopsis genus. Characteristic symptoms of guava scab are corky, ovoid or round lesions on fruit surfaces. These lesions may thicken, affecting the flesh below and reducing fruit quality and commercial value. We characterized 81 isolates isolated from guava scab lesions on guava leaves and fruit in different regions of Colombia, and identified them as Pestalotiopsis and Neopestalotiopsis spp. We analyzed the morphology, pathogenicity, and genetic diversity of the isolates based on the sequences of the internal transcribed spacer, β-tubulin, and elongation factor genes. Isolates were morphologically, pathogenically, and genetically diverse but the diversity did not correlate with geographical origin, or guava cultivar or tissue from which the isolates were recovered. Selected monosporic isolates included in the multiple-gene analysis were identified as belonging to two genera: Neopestalotiopsis (65 isolates with versicolorous conidia) and Pestalotiopsis (4 isolates with concolorous conidia).
普通番石榴（Psidium guajava L.）是一种具有全球经济重要性的果树。它因其独特的香气和风味以及营养和药用特性而在亚洲、南美和中美以及夏威夷种植。然而，番石榴生产受到番石榴疮痂病的限制，这种病是由 Pestalotiopsis 属真菌引起的。番石榴疮痂病的特征性症状是果实表面出现有弹性的、卵形或圆形的病斑。这些病斑可能会增厚，影响下面的果肉，降低果实的质量和商业价值。我们对哥伦比亚不同地区番石榴叶片和果实上的番石榴疮痂病斑中分离出的 81 个分离株进行了特征描述，鉴定为 Pestalotiopsis 和 Neopestalotiopsis 属。我们根据内转录间隔区、β-微管蛋白和延伸因子基因序列分析了分离株的形态、致病性和遗传多样性。分离株在形态、致病性和遗传上具有多样性，但这种多样性与地理起源、番石榴品种或分离株回收的组织无关。在多基因分析中包含的选定单孢分离株被鉴定为属于两个属：Neopestalotiopsis（65 个具有变色分生孢子的分离株）和 Pestalotiopsis（4 个具有同色分生孢子的分离株）。
