You Changxuan, Jin Liming, Xu Qi, Shen Bo, Jiao Xuelong, Huang Xuewu
Department of Medical Oncology, Nanfang Hospital, Guangzhou, Guangdong 510515, P.R. China.
Department of General Surgery, The Affiliated People's Hospital, Hangzhou College, Zhejiang Provincial People's Hospital, Hangzhou, Zhejiang 310014, P.R. China.
Oncol Lett. 2019 Feb;17(2):2271-2277. doi: 10.3892/ol.2018.9864. Epub 2018 Dec 21.
Effect of miR-21 and miR-138 on the proliferation of colon cancer cells and their association with prognosis were investigated. Expression levels of miR-21 and miR-138 in colorectal cancer and normal adjacent tissues were compared. Differential expression of miR-21 and miR-138 in colon cancer tissues with different clinicopathological features were analyzed. miR-21 and miR-138 expression vectors were established and transfected into cells of colon cancer cell line SW480. Methyl thiazolyl tetrazolium (MTT) assay was used to detect the proliferation of SW480 cells. Kaplan-Meier method and log-rank test were used to study the relationship between miR-21 and miR-138 expression and prognosis. Cox proportional hazards model was used to analyze the factors related to prognosis of colon cancer. Expression level of miR-21 in colon cancer tissues was significantly higher than that in adjacent tissues, and expression level of miR-138 was lower in cancer tissues than in adjacent tissues (P<0.001). Expression of miR-21 and miR-138 was associated with the degree of differentiation, lymph node metastasis, distant metastasis, and TNM stage (P<0.05). miR-21 promotes proliferation of colon cancer cell line SW480, and miR-138 inhibits cell proliferation. Survival analysis showed that the survival time of patients with high expression of miR-21 was significantly shorter than that of patients with low expression of miR-21, while survival time of patients with high expression of miR-138 was significantly longer than that of patients with low expression of miR-138 (log-rank, P<0.05). miR-21 is highly expressed in colon cancer tissues and is positively associated with the degree of malignancy of patients but negatively associated with survival. miR-138 expression is low in colon cancer tissues and is negatively associated with the degree of malignancy of patients but positively associated with survival. miR-21 and miR-138 may be involved in the regulation of colon cancer cell proliferation.
研究了miR-21和miR-138对结肠癌细胞增殖的影响及其与预后的关系。比较了结直肠癌组织和癌旁正常组织中miR-21和miR-138的表达水平。分析了miR-21和miR-138在具有不同临床病理特征的结肠癌组织中的差异表达。构建了miR-21和miR-138表达载体并转染至结肠癌细胞系SW480细胞中。采用甲基噻唑基四氮唑（MTT）法检测SW480细胞的增殖情况。采用Kaplan-Meier法和对数秩检验研究miR-21和miR-138表达与预后的关系。采用Cox比例风险模型分析结肠癌预后相关因素。结肠癌组织中miR-21的表达水平显著高于癌旁组织，而miR-138在癌组织中的表达水平低于癌旁组织（P<0.001）。miR-21和miR-138的表达与分化程度、淋巴结转移、远处转移及TNM分期相关（P<0.05）。miR-21促进结肠癌细胞系SW480的增殖，而miR-138抑制细胞增殖。生存分析显示，miR-21高表达患者的生存时间显著短于miR-21低表达患者；而miR-138高表达患者的生存时间显著长于miR-138低表达患者（对数秩检验，P<0.05）。miR-21在结肠癌组织中高表达，与患者恶性程度呈正相关，但与生存呈负相关。miR-138在结肠癌组织中表达较低，与患者恶性程度呈负相关，但与生存呈正相关。miR-21和miR-138可能参与结肠癌细胞增殖的调控。
