Nabti Larbi Zakaria, Sahli Farida, Radji Nadia, Mezaghcha Wahiba, Semara Lounis, Aberkane Salim, Lounnas Manon, Solassol Jérôme, Didelot Marie-Noelle, Jean-Pierre Hélène, Dumont Yann, Godreuil Sylvain
1 Laboratoire de Valorisation des Ressources Biologiques et Naturelles, FSNV, Université de Sétif 1, Sétif, Algérie.
2 Département des Sciences Naturelles, École Normale Supérieure Assia Djebar, Constantine, Algérie.
Microb Drug Resist. 2019 Apr;25(3):386-393. doi: 10.1089/mdr.2018.0314. Epub 2019 Jan 24.
The worldwide dissemination of multidrug-resistant (MDR) Enterobacteriaceae is a major public health issue. The aim of this study was to investigate the prevalence of MDR Escherichia coli (MDR-EC) isolates, in inpatients/outpatients with urinary tract infections at Sétif University Hospital (Algeria). Bacterial cultures were obtained from 426 of the 3,944 urine samples collected from January 2015 to February 2017. Among these cultures, 215 E. coli isolates were identified by mass spectrometry, and 38 (17.7%) were MDR-EC (disk diffusion method): 36 produced only extended-spectrum β-lactamases (ESBL), one ESBL and a carbapenemase, and one only a cephalosporinase (double-disk synergy test). Multiplex PCR and sequencing analyses showed that 37 ESBL-producing isolates harbored genes encoding CTX-M enzymes (CTX-M-15 in 33 isolates, 89.19%; and CTX-M-14 group in four isolates, 10.81%). One CTX-M-15-producing isolate co-expressed also an OXA-48-like carbapenemase. Phylogenetic group analysis of the 37 ESBL-producing and 178 non-ESBL-producing isolates indicated that the most common phylogenetic group was B2 (54.05% of ESBL-producing and 48.31% of non-ESBL-producing isolates), followed by A and D for ESBL-, and by B1, A, and F for non-ESBL-producing isolates. This is the first report highlighting the presence of MDR-EC isolates that produce both CTX-M and OXA-48-like enzymes in Sétif, Algeria.
多重耐药肠杆菌科细菌在全球范围内的传播是一个重大的公共卫生问题。本研究旨在调查阿尔及利亚塞提夫大学医院住院/门诊尿路感染患者中多重耐药大肠埃希菌（MDR-EC）分离株的流行情况。从2015年1月至2017年2月收集的3944份尿液样本中获取了426份细菌培养物。在这些培养物中，通过质谱法鉴定出215株大肠埃希菌分离株，其中38株（17.7%）为MDR-EC（纸片扩散法）：36株仅产生超广谱β-内酰胺酶（ESBL），1株产生ESBL和碳青霉烯酶，1株仅产生头孢菌素酶（双纸片协同试验）。多重PCR和测序分析表明，37株产ESBL分离株携带编码CTX-M酶的基因（33株携带CTX-M-15，占89.19%；4株携带CTX-M-14组，占10.81%）。1株产CTX-M-15的分离株还共表达了一种OXA-48样碳青霉烯酶。对37株产ESBL和178株非产ESBL分离株的系统发育群分析表明，最常见的系统发育群是B2（产ESBL分离株中占54.05%，非产ESBL分离株中占48.31%），产ESBL分离株其次是A和D群，非产ESBL分离株其次是B1、A和F群。这是首份强调在阿尔及利亚塞提夫存在同时产生CTX-M和OXA-48样酶的MDR-EC分离株的报告。
