酶介导的细胞间邻近标记检测细胞间相互作用。
Enzyme-Mediated Intercellular Proximity Labeling for Detecting Cell-Cell Interactions.
机构信息
Synthetic and Functional Biomolecules Center, Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering , Peking University , Beijing , China.
Peking-Tsinghua Center for Life Sciences , Peking University , Beijing , China.
出版信息
J Am Chem Soc. 2019 Feb 6;141(5):1833-1837. doi: 10.1021/jacs.8b10286. Epub 2019 Jan 29.
Cell-cell interactions and communications play fundamental roles in life processes but remain largely uncharacterized. We developed an enzyme-mediated proximity cell labeling (EXCELL) strategy as a general method to detect and record cell-cell interactions under living conditions. EXCELL relies on an evolved Staphylococcus aureus transpeptidase sortase A variant (mgSrtA) capable of promiscuous labeling of various cell surface proteins containing a monoglycine residue at the N-terminus. Displaying mgSrtA on the surface of a cell of interest allows the labeling and detection of interacting cells in a proximity-dependent fashion.
细胞间相互作用和通讯在生命过程中发挥着基础性作用，但很大程度上仍未被充分描述。我们开发了一种酶介导的邻近细胞标记（EXCELL）策略，作为一种在活细胞条件下检测和记录细胞间相互作用的通用方法。EXCELL 依赖于一种经过进化的金黄色葡萄球菌转肽酶 SrtA 变体（mgSrtA），该变体能够在 N 端含有单甘氨酸残基的各种细胞表面蛋白上进行混杂标记。在感兴趣的细胞表面展示 mgSrtA，可使相互作用细胞以邻近依赖性方式进行标记和检测。
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