Zafar Muhammad, Ahmad Mushtaq, Sultana Shazia, Amin Adnan, Naseer Hafiz Muhammad, Jamal Zafar, Abidin Sheikh Zain Ul, Mehmood Sajid, Khan Amir Shehzad
Department of Plant Sciences, Quaid-i-Azam University, Islamabad, Pakistan.
Department of Pharmacognosy, Faculty of Pharmacy, Gomal University, Dera Ismail Khan, Khyber Pakhtunkhwa, Pakistan.
Microsc Res Tech. 2019 May;82(5):550-557. doi: 10.1002/jemt.23199. Epub 2019 Jan 24.
The use of quality control tool for authentication of Jadwar (Delphinium denudatum Wall. ex Hook.f. & Thomson), a folk herbal drug used for the treatment of different ailments, was studied. People face problems of adulteration for this drug at global, regional, national and local levels. Two different plant species are commercially marketed in the Indo-Pak Subcontinent under the same trade name of Jadwar. One is D. denudatum Wall. ex Hook.f. & Thomson and the other is Aconitum heterophyllum Wall. ex Royle. To focus on this problem, a marketable available drug sample of Jadwar was authenticated by using basic microscopy tools (LM) and advanced chemo-taxonomic markers. Authentication, quality and standardization of this drug was achieved using morphology, organoleptography, UV and IR analyses, scanning electron microscopy of pollen and anatomical investigations. The techniques used for authentication marked the clear difference between the studied plants. Microscopic studies, chemotaxonomic investigation and other techniques used in this project provided the basis for the authentication of this species.
对用于治疗各种疾病的民间草药贾德瓦尔（Delphinium denudatum Wall. ex Hook.f. & Thomson）进行质量控制工具鉴定的研究。在全球、区域、国家和地方各级，人们都面临着这种药物掺假的问题。在印度 - 巴基斯坦次大陆，有两种不同的植物物种以相同的贾德瓦尔商品名进行商业销售。一种是Delphinium denudatum Wall. ex Hook.f. & Thomson，另一种是Aconitum heterophyllum Wall. ex Royle。为关注这一问题，通过使用基础显微镜工具（光学显微镜）和先进的化学分类标记物对市售的贾德瓦尔药物样本进行了鉴定。利用形态学、感官鉴定法、紫外和红外分析、花粉扫描电子显微镜以及解剖学研究实现了该药物的鉴定、质量控制和标准化。用于鉴定的技术明确显示了所研究植物之间的差异。本项目中使用的显微镜研究、化学分类学调查及其他技术为该物种的鉴定提供了依据。
