Laboratory of Plant Pathology, Institute of Olive Tree, Subtropical Crops and Viticulture, Hellenic Agricultural Organization Demeter (HAO-Demeter), Agrokipio 73100, Chania, Crete, Greece.
Laboratory of Wastewater Management and Treatment Technologies, Department of Environmental Engineering, Democritus University of Thrace, 67100, Xanthi, Greece.
Plant Dis. 2017 Nov;101(11):1929-1940. doi: 10.1094/PDIS-12-16-1761-RE. Epub 2017 Aug 28.
A two-year survey was conducted to identify fungi associated with wood decay in a range of tree species and grapevine. Fifty-eight fungal strains isolated from plants of 18 species showing typical wood decay symptoms were characterized by morphological, physiological, and molecular analyses. By 5.8S rRNA gene-ITS sequencing analysis, these isolates were classified into 25 distinct operational taxonomic units, including important phytopathogenic species of the phyla Pezizomycotina and Agaricomycotina, such as Fomitiporia, Inonotus, Phellinus, Inocutis, Fuscoporia, Trametes, Fusarium, Eutypa, Phaeomoniella, Phaeoacremonium, and Pleurostomophora spp. The white rot basidiomycetes Fomitiporia mediterranea (20 isolates, 34.5%) and Inonotus hispidus (6 isolates, 10.3%) were the most prevalent. Pathogenicity tests revealed for the first time that certain fungal species of the genera Fomitiporia, Inonotus, Phellinus, Pleurostomophora, and Fusarium caused wood infection of various tree species in Greece and worldwide. To the best of our knowledge, this is the first report of F. mediterranea as the causal agent of wood decay in pear, pomegranate, kumquat, and silk tree. This is also the first record of Inonotus hispidus, Phellinus pomaceus, Pleurostomophora richardsiae, and Fusarium solani in apple, almond, avocado, and mulberry tree, respectively, whereas P. richardsiae was associated with wood infection of olive tree for the first time in Greece. Cross pathogenicity tests with F. mediterranea strains originated from grapevine applied on other woody hosts and from olive on grapevine demonstrated partial host specificity of the fungus. The potential of F. mediterranea to transinfect hosts other than those originated, along with the host range extension of the fungus, is discussed.
一项为期两年的调查旨在确定与一系列树种和葡萄藤木材腐朽相关的真菌。从表现出典型木材腐朽症状的 18 个物种的植物中分离出 58 株真菌菌株，通过形态学、生理学和分子分析对其进行了特征描述。通过 5.8S rRNA 基因-ITS 测序分析，这些分离物被分为 25 个不同的操作分类单元，包括子囊菌门和担子菌门中重要的植物病原菌，如 Fomitiporia、Inonotus、Phellinus、Inocutis、Fuscoporia、Trametes、Fusarium、Eutypa、Phaeomoniella、Phaeoacremonium 和 Pleurostomophora 等属。优势白腐担子菌 Mediterranean 期 Fomitiporia（20 株，34.5%）和 Inonotus hispidus（6 株，10.3%）最为常见。致病性测试首次表明，某些属于 Fomitiporia、Inonotus、Phellinus、Pleurostomophora 和 Fusarium 的真菌物种能够引起希腊和世界各地各种树种的木材感染。据我们所知，这是首次报道 Mediterranean 期 Fomitiporia 是梨、石榴、金桔和丝棉树木材腐朽的病原体。这也是首次记录到 Inonotus hispidus、Phellinus pomaceus、Pleurostomophora richardsiae 和 Fusarium solani 分别在苹果、杏仁、鳄梨和桑树中引起木材感染，而 P. richardsiae 是首次在希腊与橄榄树的木材感染有关。对来源于葡萄藤的 Mediterranean 期 Fomitiporia 菌株与来源于橄榄的菌株在其他木本宿主上进行交叉致病性测试，表明该真菌具有部分宿主特异性。讨论了 Mediterranean 期 Fomitiporia 向非起源宿主转染的潜力以及真菌宿主范围的扩展。
