MOE International Joint Research Laboratory on Synthetic Biology and Medicines, School of Biology and Biological Engineering, South China University of Technology, Guangzhou 510006, China.
Analytical and Testing Center, South China University of Technology, Guangzhou 510640, China.
Molecules. 2020 Oct 14;25(20):4718. doi: 10.3390/molecules25204718.
A new colorimetric and fluorescence probe based on Nile-red chromophore for the detection of biothiols has been developed, exhibiting high selectivity towards biothiols over other interfering species. shows a blue shift in absorption peak upon reacting with biothiols, from 587 nm to 567 nm, which induces an obvious color change from blue to pink and exhibits a 35-fold fluorescence enhancement at 645 nm in red emission range. displays rapid (<1 min) response for HS, which is faster than other biothiols (>5 min). The detection limits of probe towards biothiols are very low (22.05 nM for HS, 34.04 nM for Cys, 107.28 nM for GSH and 113.65 nM for Hcy). Furthermore, is low cytotoxic and can be successfully applied as a bioimaging tool for real-time monitoring biothiols in HeLa cells. In addition, fluorescence mechanism of probe is further understood by theoretical calculations.
一种基于尼罗红生色团的新比色和荧光探针已被开发出来，用于检测生物硫醇，对生物硫醇具有高选择性，优于其他干扰物质。与生物硫醇反应后，吸收峰发生蓝移，从 587nm 至 567nm，这导致颜色从蓝色变为粉红色，在红色发射范围内荧光增强 35 倍，在 645nm 处发射。探针对 HS 的响应迅速（<1min），比其他生物硫醇（>5min）更快。探针对生物硫醇的检测限非常低（HS 为 22.05nM，Cys 为 34.04nM，GSH 为 107.28nM，Hcy 为 113.65nM）。此外，探针毒性低，可以成功地用作实时监测 HeLa 细胞中生物硫醇的生物成像工具。此外，通过理论计算进一步了解了探针的荧光机制。
