Giordano Mauro, Ciarambino Tiziana, D'Amico Michele, Trotta Maria Consiglia, Di Sette Alessandra Marinella, Marfella Raffaele, Malatino Lorenzo, Paolisso Giuseppe, Adinolfi Luigi Elio
Department of Advanced Medical and Surgical Sciences, University of Campania "L. Vanvitelli", 80138 Naples, Italy.
Department of Experimental Medicine, Division of Pharmacology, University of Campania "L. Vanvitelli", 80138 Naples, Italy.
J Clin Med. 2019 Jan 22;8(2):130. doi: 10.3390/jcm8020130.
We have evaluated circulating miRNAs (-195-5p and -451a) in subjects with acute ischemic stroke (AIS) and in patients with transient ischemic attack (TIA). In this study, 18 subjects with AIS and 18 patients with TIA were enrolled and examined at admission (T0) and at 24 h and 48 h after admission, and compared to 20 controls (C). At T0, circulating miRNA-195-5p and -451a were significantly upregulated in both AIS and TIA patients, compared to C. We also observed a progressive reduction of circulating miRNA levels at 24 h and 48 h in both AIS and TIA patients. Hypoxia inducible factor 1alpha (HIF-1α) serum level was significantly increased at T0, in both AIS and TIA patients, in comparison to C (both < 0.01 vs. C) and it decreased in both AIS and TIA patients at 24 h and at 48 h, in comparison to T0 (both < 0.01 vs. T0). Vascular endothelial growth factor (VEGF) serum level was significantly decreased at T0, in both AIS and TIA patients, if compared to C (both < 0.01 vs. C) and increased, in both AIS and TIA patients, at 24 h and 48 h, if compared to T0 (both < 0.01 vs. T0). The elevated expression of miRNA-195-5p and miRNA-451a significantly decreased over time at 24 h and 48 h, and it is associated with decreased HIF-α levels and increased VEGF serum levels. These data may suggest a role for this miRNAs as biomarker in the pathogenesis and prognosis of AIS patients and for the first time also in TIA patients.
我们评估了急性缺血性卒中（AIS）患者和短暂性脑缺血发作（TIA）患者体内循环miRNAs（-195-5p和-451a）的情况。在本研究中，纳入了18例AIS患者和18例TIA患者，并在入院时（T0）、入院后24小时和48小时进行检查，同时与20名对照者（C）进行比较。在T0时，与C组相比，AIS和TIA患者体内循环miRNA-195-5p和-451a均显著上调。我们还观察到，AIS和TIA患者在24小时和48小时时循环miRNA水平逐渐降低。与C组相比，AIS和TIA患者在T0时缺氧诱导因子1α（HIF-1α）血清水平显著升高（两者均P<0.01 vs. C），而与T0时相比，AIS和TIA患者在24小时和48小时时该水平均降低（两者均P<0.01 vs. T0）。与C组相比，AIS和TIA患者在T0时血管内皮生长因子（VEGF）血清水平显著降低（两者均P<0.01 vs. C），而与T0时相比，AIS和TIA患者在24小时和48小时时该水平升高（两者均P<0.01 vs. T0）。miRNA-195-5p和miRNA-451a的表达升高在24小时和48小时时随时间显著降低，且与HIF-α水平降低和VEGF血清水平升高相关。这些数据可能提示这些miRNAs在AIS患者的发病机制和预后中作为生物标志物发挥作用，并且首次表明在TIA患者中也如此。
