Cernencu Alexandra I, Vlasceanu George M, Serafim Andrada, Pircalabioru Gratiela, Ionita Mariana
Advanced Polymer Materials Group, University Politehnica of Bucharest 1-7 Gh. Polizu Street Bucharest 011061 Romania
Faculty of Medical Engineering, University Politehnica of Bucharest 1-7 Gh. Polizu Street Bucharest 011061 Romania
RSC Adv. 2023 Aug 10;13(34):24053-24063. doi: 10.1039/d3ra02786d. eCollection 2023 Aug 4.
The advent of improved fabrication technologies, particularly 3D printing, has enabled the engineering of bone tissue for patient-specific healing and the fabrication of tissue models for testing. However, inks made from natural polymers often fall short in terms of mechanical strength, stability, and the induction of osteogenesis. Our research focused on developing novel printable formulations using a gelatin/pectin polymeric matrix that integrate synergistic reinforcement components graphene oxide (GO) and oxidized nanocellulose fibers (CNF). Using 3D printing technology and the aforementioned biomaterial composite inks, bone-like scaffolds were created. To simulate critical-sized flaws and demonstrate scaffold fidelity, 3D scaffolds were successfully printed using formulations with varied GO concentrations (0.25, 0.5, and 1% wt with respect to polymer content). The addition of GO to hydrogel inks enhanced not only the compressive modulus but also the printability and scaffold fidelity compared to the pure colloid-gelatin/pectin system. Due to its strong potential for 3D bioprinting, the sample containing 0.5% GO is shown to have the greatest perspectives for bone tissue models and tissue engineering applications.
改进的制造技术的出现，特别是3D打印技术，使得针对患者特异性愈合的骨组织工程以及用于测试的组织模型制造成为可能。然而，由天然聚合物制成的墨水在机械强度、稳定性和成骨诱导方面往往存在不足。我们的研究重点是开发一种新型的可打印配方，使用明胶/果胶聚合物基质，并整合具有协同增强作用的成分——氧化石墨烯（GO）和氧化纳米纤维素纤维（CNF）。利用3D打印技术和上述生物材料复合墨水，制造出了类骨支架。为了模拟临界尺寸缺陷并展示支架的逼真度，使用了具有不同GO浓度（相对于聚合物含量为0.25%、0.5%和1%重量）的配方成功打印出了3D支架。与纯胶体 - 明胶/果胶体系相比，向水凝胶墨水中添加GO不仅提高了压缩模量，还增强了可打印性和支架逼真度。由于其在3D生物打印方面具有强大潜力，含有0.5% GO的样品在骨组织模型和组织工程应用方面显示出最大的前景。
