Department of Pharmaceutical Chemistry, College of Health Sciences, University of KwaZulu-Natal, Durban 4000, South Africa.
Department of Pharmaceutical Chemistry, College of Health Sciences, University of KwaZulu-Natal, Durban 4000, South Africa; Department of Applied Sciences, Women Institute of Technology, Dehradun 248007, India.
Mater Sci Eng C Mater Biol Appl. 2019 Apr;97:285-292. doi: 10.1016/j.msec.2018.12.018. Epub 2018 Dec 7.
A glassy carbon electrode modified with electrochemically polymerized methyl orange (PMO) and multi-walled carbon nanotubes (MWCNT) was developed. The morphologies of the fabricating materials (PMO and MWCNT) were investigated by field-emission scanning electron microscopy (FE-SEM). The designed sensor was used for the sensitive determination of amodiaquine (AQ), an anti-malaria drug. AQ was developed as an alternative to chloroquine because of its activity against chloroquine-resistant Plasmodium falciparum (P. falciparum) parasites. The modified electrode was employed to study the electrochemical oxidation of AQ using cyclic voltammetry (CV) and differential pulse voltammetry (DPV) techniques. Under optimal experimental conditions, DPV exhibited a linear response in the concentration range from 1.0 × 10 to 3.5 × 10 mol L with a limit of detection (LOD) of 8.9 × 10 mol L. Furthermore, the number of electrons and protons involved in the electrochemical study of AQ was also calculated and a plausible mechanism for the electro-oxidation of AQ was deduced. The developed sensor demonstrated analytical applicability as it was successfully employed to determine the drug AQ in pharmaceutical formulations and human urine samples.
研制了一种经电化学聚合甲基橙（PMO）和多壁碳纳米管（MWCNT）修饰的玻碳电极。采用场发射扫描电子显微镜（FE-SEM）对制备材料（PMO 和 MWCNT）的形态进行了研究。该设计的传感器用于灵敏测定抗疟药物阿莫地喹（AQ）。由于其对氯喹耐药的恶性疟原虫（P. falciparum）寄生虫具有活性，AQ 被开发为氯喹的替代品。使用循环伏安法（CV）和差分脉冲伏安法（DPV）技术，在优化实验条件下，修饰电极研究了 AQ 的电化学氧化。在最佳实验条件下，DPV 在 1.0×10 到 3.5×10 mol L 的浓度范围内呈现线性响应，检测限（LOD）为 8.9×10 mol L。此外，还计算了 AQ 电化学研究中涉及的电子和质子数量，并推导出 AQ 电氧化的合理机制。所开发的传感器具有分析适用性，因为它已成功用于测定药物制剂和人尿样中的 AQ 药物。
