São Paulo State University (UNESP), Institute of Chemistry, Araraquara, SP, Brazil.
São Paulo State University (UNESP), Faculty of Agricultural Sciences, Botucatu, SP, Brazil.
Mater Sci Eng C Mater Biol Appl. 2019 Apr;97:576-582. doi: 10.1016/j.msec.2018.12.042. Epub 2018 Dec 13.
Natural Rubber Latex (NRL) is a biocompatible material with demonstrated capacity to induce vascularisation and tissue regeneration. Propolis is a complex resinous product prepared by Apis mellifera with the aim of protecting beehives against infectious microorganisms. It is flora-dependent and its antimicrobial activity can vary according to its geographical origin. This study compares the incorporation of three different types of propolis into an NRL membrane aiming at optimal controlled release of propolis potential antimicrobial compounds towards Candida albicans whilst keeping NRL mechanical characteristics desirable for wound healing bandage purposes. The propolis samples were classified as red, green and poplar propolis according to their chemical composition determined by ultra-high performance liquid chromatography coupled in series with both UV spectrophotometry and high-resolution mass spectrometry. The Minimum Inhibitory Concentrations (MIC) towards C. albicans were determined before their incorporation into NRL membranes. The release of NRL-propolis components in Simulated Body Fluid (SBF) was monitored by UV-Vis spectroscopy. The antimicrobial activity and the effects of the materials released on mouse fibroblasts were assessed. FTIR analyses were carried out in order to verify the formation of new chemical bonds that might prevent the release of propolis components from the NRL membrane. The mechanical characteristics of the NRL membranes remained adequate after the incorporation of the three types of propolis investigated whilst allowing the release of the red, and poplar propolis most active compounds against C. albicans. At 30 and 50% the released materials (eluates) from the NRL membranes incorporated with red and poplar propolis types were not toxic to fibroblast cells. These results suggest that red and poplar propolis can be incorporated into NRL membranes for the preparation of wound healing dressing.
天然胶乳（NRL）是一种具有生物相容性的材料，已被证明具有诱导血管生成和组织再生的能力。蜂胶是由蜜蜂为保护蜂巢免受感染性微生物而产生的一种复杂的树脂状产物。它依赖于植物，其抗菌活性会因其地理来源而有所不同。本研究比较了三种不同类型的蜂胶掺入 NRL 膜中，旨在实现蜂胶潜在抗菌化合物对白色念珠菌的最佳控制释放，同时保持 NRL 对伤口愈合绷带所需的机械特性。根据超高效液相色谱法与紫外分光光度法和高分辨率质谱法串联测定的蜂胶化学成分，将蜂胶样品分为红蜂胶、绿蜂胶和杨蜂胶。在将其掺入 NRL 膜之前，测定了其对白色念珠菌的最小抑菌浓度（MIC）。通过紫外可见光谱监测 NRL-蜂胶成分在模拟体液（SBF）中的释放情况。评估了材料的抗菌活性及其释放物对小鼠成纤维细胞的影响。进行了傅里叶变换红外（FTIR）分析，以验证可能阻止蜂胶成分从 NRL 膜中释放的新化学键的形成。在所研究的三种类型的蜂胶掺入后，NRL 膜的机械特性仍然足够，同时允许释放对白色念珠菌最具活性的红蜂胶和杨蜂胶化合物。在 30%和 50%时，掺入红蜂胶和杨蜂胶类型的 NRL 膜的释放材料（洗脱液）对成纤维细胞无毒。这些结果表明，红蜂胶和杨蜂胶可以掺入 NRL 膜中制备伤口愈合敷料。
