Zvěřová Martina, Hroudová Jana, Fišar Zdeněk, Hansíková Hana, Kališová Lucie, Kitzlerová Eva, Lambertová Alena, Raboch Jiří
Department of Psychiatry, First Faculty of Medicine, Charles University and General University Hospital in Prague, 120 00 Prague 2, Czech Republic,
Institute of Pharmacology, First Faculty of Medicine, Charles University and General University Hospital in Prague, 128 00 Prague 2, Czech Republic,
Neuropsychiatr Dis Treat. 2019 Jan 14;15:233-240. doi: 10.2147/NDT.S188964. eCollection 2019.
Mitochondrial dysfunctions are implicated in the pathophysiology of mood disorders. We measured and examined the following selected mitochondrial parameters: citrate synthase (CS) activity, electron transport system (ETS) complex (complexes I, II, and IV) activities, and mitochondrial respiration in blood platelets.
The analyses were performed for 24 patients suffering from a depressive episode of bipolar affective disorder (BD), compared to 68 patients with MDD and 104 healthy controls. BD and unipolar depression were clinically evaluated using well-established diagnostic scales and questionnaires.
The CS, complex II, and complex IV activities were decreased in the depressive episode of BD patients; complex I and complex I/CS ratio were significantly increased compared to healthy controls. We observed significantly decreased complex II and CS activities in patients suffering from MDD compared to controls. Decreased respiration after complex I inhibition and increased residual respiration were found in depressive BD patients compared to controls. Physiological respiration and capacity of the ETS were decreased, and respiration after complex I inhibition was increased in MDD patients, compared to controls. Increased complex I activity can be a compensatory mechanism for decreased CS and complex II and IV activities.
We can conclude that complex I and its abnormal activity contribute to the defects in cellular energy metabolism during a depressive episode of BD. The observed parameters could be used in a panel of biomarkers that could selectively distinguish BD depression from MDD and can be easily examined from blood elements.
线粒体功能障碍与情绪障碍的病理生理学有关。我们测量并检查了以下选定的线粒体参数：柠檬酸合酶（CS）活性、电子传递系统（ETS）复合物（复合物I、II和IV）活性以及血小板中的线粒体呼吸。
对24例双相情感障碍（BD）抑郁发作患者进行分析，并与68例重度抑郁症（MDD）患者和104例健康对照进行比较。使用成熟的诊断量表和问卷对BD和单相抑郁症进行临床评估。
BD患者抑郁发作时CS、复合物II和复合物IV活性降低；与健康对照相比，复合物I和复合物I/CS比值显著升高。与对照组相比，我们观察到MDD患者的复合物II和CS活性显著降低。与对照组相比，抑郁BD患者在复合物I抑制后呼吸显著降低，残余呼吸增加。与对照组相比，MDD患者的生理呼吸和ETS能力降低，复合物I抑制后的呼吸增加。复合物I活性增加可能是CS、复合物II和IV活性降低的一种代偿机制。
我们可以得出结论，复合物I及其异常活性导致BD抑郁发作期间细胞能量代谢缺陷。观察到的参数可用于一组生物标志物，这些生物标志物可以选择性地将BD抑郁症与MDD区分开来，并且可以很容易地从血液成分中进行检测。
