Zhou Jianping, Shi Jun, Fu Xingli, Mao Boneng, Wang Weimin, Li Weiling, Li Gang, Zhou Sujun
Department of General Surgery, Yixing People's Hospital The Affiliated Hospital of Jiangsu University, Yixing 214200, Jiangsu, P.R China.
Health Science Center, Jiangsu University, Zhenjiang 212000, Jiangsu, P.R China.
Oncotarget. 2018 Jan 3;9(101):37471-37479. doi: 10.18632/oncotarget.23928. eCollection 2018 Dec 25.
Recent studies revealed that several Long noncoding RNAs (LncRNAs) are associated with progression of gastric cancer (GC), while the functional role and molecular mechanism of many GC-associated lncRNAs remain undetermined. The tumor suppressor-gene retinoblastoma gene (RB1) was decreased in several human cancers including gastric cancer (GC). In this study, we investigated whether Linc00441 was involved in the suppression of RB1. Our findings showed that the up-regulated Linc00441 was inversely correlated with RB1 expression in human GC tumor samples. The gain- and loss-of-function investigation revealed that Linc00441 could promote the proliferation of GC cells. Furthermore, RNA pull down and RIP assays demonstrated that Linc00441 could recruit DNMT1 to the RB1 promoter and suppressed RB1 expression in GC cells. In conclusion, our findings revealed that Linc00441 played crucial role in GC progression and suggested that Linc00441 was potentially an effective target for GC therapy in the future.
最近的研究表明，几种长链非编码RNA（lncRNAs）与胃癌（GC）的进展相关，而许多与GC相关的lncRNAs的功能作用和分子机制仍未确定。肿瘤抑制基因视网膜母细胞瘤基因（RB1）在包括胃癌（GC）在内的几种人类癌症中表达降低。在本研究中，我们调查了Linc00441是否参与了RB1的抑制作用。我们的研究结果表明，在人GC肿瘤样本中，上调的Linc00441与RB1表达呈负相关。功能获得和功能缺失研究表明，Linc00441可促进GC细胞的增殖。此外，RNA下拉和RIP分析表明，Linc00441可将DNMT1募集到RB1启动子，并抑制GC细胞中RB1的表达。总之，我们的研究结果表明Linc00441在GC进展中起关键作用，并表明Linc00441可能是未来GC治疗的有效靶点。
