Afroozi Bahar, Mardani Maryam, Motaghi Ahmad, Tahmorespour Arezoo
Postgraduate Student, Dept. of Oral and Maxillofacial Medicine, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.
Dept. of Oral and Maxillofacial Medicine, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.
J Dent (Shiraz). 2018 Dec;19(4):305-310.
Due to the close contact with patients during dental treatments, dentists and those affiliated to the dental profession are at higher risk for various infections. Infection prevention in dentistry is an important topic that has gained more interest in recent years.
We aimed to evaluate the protective role of front-closed and front-open gowns against staphylococcus aureus contamination of dental students before and after restorative treatments.
Sixty male dental students performed the restorative treatments on the teeth of the patients in the front-closed and front-open gowns groups. Before and after the treatment, the wet sterile swab samples were collected from the students' neck and anterior part of the chest. The samples simultaneously cultured on the blood agar and the Staphylococcus-specific medium using spread plate method. Finally, the colonies were counted within 24-48 hours.
The mean of the total colony count increased in both groups of front-open (787.8 ± 88.91) and front-closed gowns (630 ±122.7), but the changes were significant only in the front-open gown group (≤ 0.001). Compared to the front-closed gown group (430±71.08), the total colony count of in the front-open gown group (490.3±62.5) was increased significantly (≤ 0.001).
We confirmed that dental students occupationally exposed to the bacterial agents and even simple minor changes in gown could considerably decrease the contamination. Education about bacterial transmission, as well as infection prevention and control measures is necessary for dental students, especially when they participate in clinical practice.
由于在牙科治疗过程中与患者密切接触,牙医及牙科行业相关人员面临各种感染的风险更高。牙科感染预防是近年来备受关注的重要课题。
我们旨在评估前开式和前闭式手术衣对牙科学生在修复治疗前后金黄色葡萄球菌污染的防护作用。
60名男性牙科学生分别穿着前开式和前闭式手术衣为患者进行牙齿修复治疗。治疗前后,用湿无菌拭子从学生的颈部和胸部前方采集样本。样本采用涂布平板法同时接种于血琼脂和葡萄球菌特异性培养基上。最后,在24 - 48小时内对菌落进行计数。
前开式手术衣组(787.8 ± 88.91)和前闭式手术衣组(630 ± 122.7)的总菌落数平均值均有所增加,但仅前开式手术衣组的变化具有统计学意义(≤ 0.001)。与前闭式手术衣组(430 ± 71.08)相比,前开式手术衣组的总菌落数(490.3 ± 62.5)显著增加(≤ 0.001)。
我们证实牙科学生职业性接触细菌病原体,即使手术衣的简单微小变化也可能显著降低污染。对牙科学生进行细菌传播以及感染预防和控制措施的教育是必要的,尤其是当他们参与临床实践时。