Devang Divakar Darshan, Aldeyab Sultan Salem, Alfawaz Sara A, AlKheraif Abdulaziz Abdullah, Ahmed Khan Aftab
Dental Biomaterials Research Chair, Dental Health Department, College of Applied Medical Sciences, King Saud University, Riyadh 11433, Saudi Arabia; Department of Oral Medicine and Radiology, KVG Dental College and Hospital, Sullia, Karnataka, India.
Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, PO Box 2435, University of Dammam, Dammam 31441, Saudi Arabia.
Microb Pathog. 2017 Aug;109:29-34. doi: 10.1016/j.micpath.2017.05.017. Epub 2017 May 12.
Staphylococcus epidermidis is one of most prevalent in dental caries or dental pulp which has the capability of horizontal genetic transfer between different bacterial species in the oropharynx, suggesting that it may evolve with the dissemination of resistant determinants, This study was performed to molecularly characterize and differentiate S. epidermidis isolated from dental caries and healthy individual. Also, two important cytokines in inflammation were assayed caused due to S. epidermidis of health and dental caries sources. Dental caries strains were more resistant with high MIC and MIC value. These isolates also showed the presence of mecA gene and another virulence gene i. e sea and seb comparatively more than healthy individual isolates. SCCmec types, III and IV was more prevalent in dental caries isolates where an as healthy individual was more non-typable. Additionally, the quantity of IL-1β and IL-8 caused due to dental caries isolates was seen more which indicate dental caries isolates are able to induce. This study showed that S. epidermidis a normal flora of oropharyngeal are more diverse to those strains which cause dental caries. S. epidermidis owns a prodigious genetic plasticity that permits to obtain, lose or regulate genetic elements that provide compensations to improve its colonization in the host.
表皮葡萄球菌是龋齿或牙髓中最常见的细菌之一，它能够在口咽中的不同细菌物种之间进行水平基因转移，这表明它可能随着耐药决定因素的传播而进化。本研究旨在对从龋齿和健康个体中分离出的表皮葡萄球菌进行分子特征分析和鉴别。此外，还检测了健康和龋齿来源的表皮葡萄球菌引起的炎症中的两种重要细胞因子。龋齿菌株对高MIC和MIC值的耐药性更强。与健康个体分离株相比，这些分离株还显示出mecA基因以及另一种毒力基因即sea和seb的存在。III型和IV型SCCmec在龋齿分离株中更为普遍，而健康个体的分离株则更难以分型。此外，龋齿分离株引起的IL-1β和IL-8的量更多，这表明龋齿分离株能够诱导产生。这项研究表明，作为口咽正常菌群的表皮葡萄球菌与那些引起龋齿的菌株相比更加多样化。表皮葡萄球菌具有巨大的遗传可塑性，这使得它能够获得、丢失或调节那些提供补偿以改善其在宿主体内定殖的遗传元件。
