Beijing Bioprocess Key Laboratory, State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, 100029, People's Republic of China.
Amoy - BUCT Industrial Bio-technovation Institute, Amoy, 361022, People's Republic of China.
Appl Biochem Biotechnol. 2019 Jul;188(3):741-749. doi: 10.1007/s12010-018-02948-1. Epub 2019 Jan 25.
Cellulase is an important enzyme that can be used to breakdown lignocellulose into glucose. Microbulbifer hydrolyticus IRE-31(ATCC 700072) is a kind of marine bacterium, which could grow in high salinity medium and has fast-strong growth ability. In this study, a novel strain was screened from Microbulbifer hydrolyticus IRE-31 through mutations to produce cellulase. The effect of different carbon sources on the growth as well as on the production of cellulase of the new strain was studied. Carboxymethyl-cellulase (CMCase) activity selected to represent cellulase was proven to be effectively promoted while xylose, galactose, and melibiose as well as glucose were used as carbon sources. When xylose and glucose were chosen to be further investigated, 472.57 U/L and 266.01 U/L CMCase activity were obtained from 30 g/L glucose and 10 g/L xylose, respectively. These results clarified the effect of different carbon sources on the production of cellulase, which laid a good foundation for the further research in the production of cellulase by marine bacteria.
纤维素酶是一种重要的酶，可以将木质纤维素分解为葡萄糖。海洋细菌微菌属水解菌 IRE-31（ATCC 700072）能够在高盐培养基中生长，具有快速生长的能力。本研究通过诱变从微菌属水解菌 IRE-31 中筛选出一种新型菌株来生产纤维素酶。研究了不同碳源对新菌株生长和纤维素酶生产的影响。选择羧甲基纤维素酶（CMCase）活性来代表纤维素酶，结果表明，当使用木糖、半乳糖、棉子糖和葡萄糖作为碳源时，CMCase 活性得到了有效促进。当进一步选择木糖和葡萄糖作为碳源时，从 30 g/L 葡萄糖和 10 g/L 木糖中分别获得了 472.57 U/L 和 266.01 U/L 的 CMCase 活性。这些结果阐明了不同碳源对纤维素酶生产的影响，为进一步研究海洋细菌生产纤维素酶奠定了良好的基础。
