Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, People's Republic of China.
Appl Biochem Biotechnol. 2012 Aug;167(7):1963-72. doi: 10.1007/s12010-012-9734-4. Epub 2012 May 27.
The components of a natural medium were optimized to produce cellulase from a marine Aspergillus niger under solid state fermentation conditions by response surface methodology. Eichhornia crassipes and natural seawater were used as a major substrate and a source of mineral salts, respectively. Mineral salts of natural seawater could increase cellulase production. Raw corn cob and raw rice straw showed a significant positive effect on cellulase production. The optimum natural medium consisted of 76.9 % E. crassipes (w/w), 8.9 % raw corn cob (w/w), 3.5 % raw rice straw (w/w), 10.7 % raw wheat bran (w/w), and natural seawater (2.33 times the weight of the dry substrates). Incubation for 96 h in the natural medium increased the biomass to the maximum. The cellulase production was 17.80 U/g the dry weight of substrates after incubation for 144 h. The natural medium avoided supplying chemicals and pretreating substrates. It is promising for future practical fermentation of environment-friendly producing cellulase.
采用响应面法优化天然培养基成分，在固态发酵条件下由海洋黑曲霉生产纤维素酶。凤眼蓝和天然海水分别用作主要底物和无机盐源。天然海水的无机盐可以提高纤维素酶的产量。生玉米芯和生稻草对纤维素酶的生产有显著的积极影响。最佳的天然培养基由 76.9%的凤眼蓝（w/w）、8.9%的生玉米芯（w/w）、3.5%的生稻草（w/w）、10.7%的生麦麸（w/w）和天然海水（干底物重量的 2.33 倍）组成。在天然培养基中培养 96 h 可将生物量提高到最大值。在 144 h 的培养后，纤维素酶的产量为每克干底物 17.80 U。该天然培养基避免了化学物质的供应和底物的预处理。它有望在未来用于环保生产纤维素酶的实际发酵中。
