Vega-Lopez F, Stoker N G, Locniskar M F, Dockrell H M, Grant K A, McAdam K P
Department of Clinical Sciences, London School of Hygiene and Tropical Medicine, England.
J Clin Microbiol. 1988 Dec;26(12):2474-9. doi: 10.1128/jcm.26.12.2474-2479.1988.
Mycobacterium leprae sonic extracts prepared from armadillo-derived bacteria were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting (immunoblotting) procedures and probed with serum or plasma samples from 20 patients with lepromatous leprosy and 14 healthy endemic controls. Five proteins of 33, 25, 18, 15, and 12 kilodaltons (kDa) were frequently recognized; the 33- and 15-kDa proteins were, respectively, recognized with high intensity by 16 and 13 of the 20 samples from patients with leprosy, whereas only one healthy donor had antibodies that recognized the 15-kDa protein. By the use of M. leprae-specific murine monoclonal antibodies it was demonstrated that the 33-, 25-, and 15-kDa antigens were different from those bound by the available murine monoclonal antibodies. The 18- and 12-kDa proteins detected had molecular masses similar to those detected by the corresponding murine monoclonal antibodies. The serum and plasma samples from patients with leprosy were also used to probe Western blots of a soluble extract of M. tuberculosis. They recognized, among others, antigens with molecular weights similar to those detected in the M. leprae antigenic preparations, although with less intensity and at a lower frequency.
对从犰狳源细菌制备的麻风分枝杆菌超声提取物进行十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和蛋白质印迹（免疫印迹）分析，并用20例瘤型麻风患者和14名健康流行区对照者的血清或血浆样本进行检测。经常能识别出分子量为33、25、18、15和12千道尔顿（kDa）的5种蛋白质；20例麻风患者样本中的16例和13例分别高强度识别出33 kDa和15 kDa的蛋白质，而只有1名健康供体有识别15 kDa蛋白质的抗体。通过使用麻风分枝杆菌特异性鼠单克隆抗体证明，33 kDa、25 kDa和15 kDa抗原与现有鼠单克隆抗体结合的抗原不同。检测到的18 kDa和12 kDa蛋白质的分子量与相应鼠单克隆抗体检测到的分子量相似。麻风患者的血清和血浆样本也用于检测结核分枝杆菌可溶性提取物的蛋白质印迹。除其他外，他们识别出分子量与麻风分枝杆菌抗原制剂中检测到的分子量相似的抗原，尽管强度较低且频率较低。
