Pessolani M C, Rumjanek F D, Marques M A, de Melo F S, Sarno E N
Setor de Hanseníase, Fundacäo Oswaldo Cruz, Rio de Janeiro, Brazil.
J Clin Microbiol. 1989 Oct;27(10):2184-9. doi: 10.1128/jcm.27.10.2184-2189.1989.
Analysis by Western immunoblotting of Mycobacterium bovis BCG short-term culture filtrates with a pool of serum samples from lepromatous leprosy patients revealed an immunodominant protein doublet with apparent molecular masses of 28 and 30 kilodaltons (kDa). The humoral response to these antigens was also investigated by using individual serum samples from patients representative of the whole spectrum of leprosy and from tuberculosis patients, as well as from contacts of leprosy patients and control groups. The protein doublet was recognized by 92% of the sera from patients with lepromatous leprosy (51 of 56), whereas essentially negative results were obtained with sera from the other groups. Similar immunodominant bands were also detected by Western blotting analysis of sonic extracts of seven other slow- and fast-growing mycobacterial species, suggesting a broad distribution of these antigens within the genus. Analysis of the purified doublet by Western blotting after two-dimensional gel electrophoresis fractionation showed that the 28- and 30-kDa doublet consisted of at least five different components with pls from 5.2 to 5.7 and molecular masses from 28 to 31 kDa. These results indicate that the protein doublet could be used as a potential marker in the diagnosis of lepromatous leprosy.
用瘤型麻风患者血清样本池对牛分枝杆菌卡介苗短期培养滤液进行蛋白质免疫印迹分析，发现一种免疫显性蛋白双峰，其表观分子量为28和30千道尔顿（kDa）。还使用来自代表整个麻风病谱的患者、结核病患者以及麻风病患者接触者和对照组的个体血清样本，研究了对这些抗原的体液反应。56份瘤型麻风患者血清中有51份（92%）识别出该蛋白双峰，而其他组血清基本呈阴性结果。对其他七种快速和慢速生长分枝杆菌物种的超声提取物进行蛋白质免疫印迹分析，也检测到类似的免疫显性条带，表明这些抗原在该属内广泛分布。二维凝胶电泳分离后对纯化的双峰进行蛋白质免疫印迹分析表明，28 kDa和30 kDa的双峰至少由五种不同成分组成，其等电点为5.2至5.7，分子量为28至31 kDa。这些结果表明，该蛋白双峰可作为瘤型麻风诊断的潜在标志物。
