Identification and Validation of Reference Genes for Gene Expression Analysis in .

Real-time quantitative PCR (RT-qPCR) is a reliable and high-throughput technique for gene expression studies, but its accuracy depends on the expression stability of reference genes. is a fast-growing timber species with strong resistance. However, thus far, reliable reference gene identifications have not been reported in . In this study, 19 candidate reference genes were selected and evaluated for their expression stability in different tissues of . Three software programs (geNorm, NormFinder, and BestKeeper) were used to evaluate the reference gene transcript stabilities, and comprehensive stability ranking was generated by the geometric mean method. Our results show that was the most stable reference gene and that + was the best reference gene combination for different tissues. Finally, the stable and less stable reference genes were verified using expression in different tissues. To our knowledge, this is the first report to verify appropriate reference genes for normalizing gene expression in for different tissues, which will facilitate the future elucidation of gene regulations in this species and useful references for relative species.