Peachey L D, Eisenberg B R
Biophys J. 1978 May;22(2):145-54. doi: 10.1016/S0006-3495(78)85480-0.
Reconstruction from thick serial transverse slices of frog skeletal muscle fibers stained with peroxidase and examined by high-voltage electron microscopy has revealed that the T system networks at successive sarcomeres are connected together in a helicoidal fashion. From zero to eight helicoids have been found in each of a group of 21 fibers reconstructed in cross section. Helicoids can have either right- or left-handed screw senses, and both senses can be found in one fiber cross section. Because the T system maintains a relatively precise alignment with the myofibrillar striations, it follows that the striations must also have a helicoidal arrangement. This has been found before, but has not been widely accepted in recent times. The presence of helicoids in the bands and membrane networks is not thought per se to alter very much our thinking about excitation and contraction mechanisms in skeletal muscle fibers.
对用过氧化物酶染色并通过高压电子显微镜检查的青蛙骨骼肌纤维的厚连续横向切片进行重建后发现，连续肌节处的T系统网络以螺旋方式连接在一起。在一组21根重建横截面的纤维中，每根纤维都发现有0至8个螺旋。螺旋可以有右手或左手螺旋方向，并且在一个纤维横截面中可以同时发现这两种方向。由于T系统与肌原纤维条纹保持相对精确的对齐，因此条纹也必定具有螺旋排列。这一点以前就已发现，但近年来尚未被广泛接受。带和膜网络中螺旋的存在本身并不被认为会极大地改变我们对骨骼肌纤维兴奋和收缩机制的看法。
