Biomaterials Innovation Research Center, Division of Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts.
Harvard-Massachusetts Institute of Technology, Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, Massachusetts.
J Biomed Mater Res A. 2019 Jun;107(6):1225-1234. doi: 10.1002/jbm.a.36622. Epub 2019 Feb 18.
Due to the limited success rate of currently available vascular replacements, tissue engineering has received tremendous attention in recent years. A main challenge in the field of regenerative medicine is creating a mechanically functional tissue with a well-organized extracellular matrix, particularly of collagen and elastin. In this study, the native collagen scaffold derived from decellularized tendon sections, as a scaffold having the potential to be used for vascular tissue engineering applications, was studied. We showed that the elasticity of the scaffolds was improved when crosslinked with the bovine elastin. The effect of different concentrations of elastin on mechanical properties of the collagen scaffolds was evaluated of which 15% elastin concentration was selected for further analysis based on the results. Addition of 15% elastin to collagen scaffolds significantly decreased Young's modulus and the tensile stress at the maximum load and increased the tensile strain at the maximum load of the constructs as compared to those of the collagen scaffolds or control samples. Moreover, tubular elastin modified collagen scaffolds showed significantly higher burst pressure compared to the control samples. Smooth muscle cells and endothelial cells cultured on the elastin modified collagen scaffolds showed high viability (>80%) after 1, 3, and 7 days. Furthermore, the cells showed a high tendency to align with the collagen fibers within the scaffold and produced their own extracellular matrix over time. In conclusion, the results show that the decellularized tendon sections have a great potential to be used as scaffolds for vascular tissue engineering applications. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 1225-1234, 2019.
由于目前可用的血管替代品的成功率有限，组织工程近年来受到了极大的关注。再生医学领域的一个主要挑战是创建具有组织良好的细胞外基质（尤其是胶原蛋白和弹性蛋白）的机械功能组织。在这项研究中，研究了源自脱细胞肌腱部分的天然胶原蛋白支架，作为一种具有用于血管组织工程应用潜力的支架。我们表明，用牛弹性蛋白交联时，支架的弹性得到了改善。评估了不同浓度的弹性蛋白对胶原蛋白支架机械性能的影响，根据结果选择了 15%的弹性蛋白浓度进行进一步分析。与胶原蛋白支架或对照样品相比，向胶原蛋白支架中添加 15%的弹性蛋白可显著降低杨氏模量和最大载荷下的拉伸应力，并增加最大载荷下的拉伸应变。此外，与对照样品相比，管状弹性蛋白修饰的胶原蛋白支架的爆破压力明显更高。在弹性蛋白修饰的胶原蛋白支架上培养的平滑肌细胞和内皮细胞在第 1、3 和 7 天时的存活率均高于 80%。此外，随着时间的推移，这些细胞表现出与支架内的胶原蛋白纤维高度对齐的趋势，并产生了自己的细胞外基质。总之，结果表明脱细胞肌腱部分具有作为血管组织工程应用支架的巨大潜力。© 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part A：107A：1225-1234，2019。
