Center for Eukaryotic Gene Regulation, Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802, USA; The Huck Institutes of Life Sciences, The Pennsylvania State University, University Park, PA 16802, USA.
Center for Eukaryotic Gene Regulation, Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802, USA.
Methods. 2019 Apr 15;159-160:129-137. doi: 10.1016/j.ymeth.2019.01.010. Epub 2019 Jan 24.
The Carboxy-terminal Domain (CTD) of RNA polymerase II (Pol II) plays essential roles in regulating gene expression in eukaryotes. Here, we describe multiple genetic approaches for studying the CTD in Drosophila that complement pre-existing molecular analyses of the Pol II CTD in other experimental models. These approaches will allow one to assess the effects of any CTD mutations in a developmentally complex organism. The approaches discussed in this work can in principle, be applied to analyze other transcription components in eukaryotes.
RNA 聚合酶 II（Pol II）的羧基末端结构域（CTD）在真核生物中调节基因表达中发挥着重要作用。在这里，我们描述了多种用于研究果蝇 CTD 的遗传方法，这些方法补充了其他实验模型中 Pol II CTD 的分子分析。这些方法将允许人们在发育复杂的生物体中评估任何 CTD 突变的影响。本文讨论的方法原则上可以应用于分析真核生物中的其他转录成分。
