Clinic for Pediatric Pneumology, Allergology and Neonatology, Hannover Medical School, D-30625 Hannover, Germany.
Clinic for Pediatric Pneumology, Allergology and Neonatology, Hannover Medical School, D-30625 Hannover, Germany; Reseach Core Unit Genomics, Hannover Medical School, D-30625 Hannover, Germany.
J Cyst Fibros. 2019 Sep;18(5):653-656. doi: 10.1016/j.jcf.2019.01.003. Epub 2019 Jan 23.
Bacterial replication rates were determined from metagenome sequencing of nasal lavage, throat swabs and induced sputa collected from healthy subjects and individuals with COPD or cystic fibrosis. More than 90% of peak-to-trough coverage ratios of major clones were above 1.4 indicating that the most abundant bacterial species in the microbial communities were replicating in the airways including common inhabitants such as Prevotella and Streptococcus species as well as the cystic fibrosis pathogens Staphylococcus aureus and Pseudomonas aeruginosa. The populations of P. aeruginosa and S. aureus were replicating their pool of chromosomes more slowly than the populations of the common inhabitants of a healthy airway microbial flora. The assessment of growth dynamics in microbial metagenomes could become a decision-making tool for the diagnosis and management of bacterial infections in cystic fibrosis.
细菌复制率是通过对健康受试者和 COPD 或囊性纤维化患者的鼻洗液、咽喉拭子和诱导痰进行宏基因组测序来确定的。主要克隆的峰谷覆盖率超过 90%，这表明微生物群落中最丰富的细菌物种在气道中复制，包括常见的普雷沃氏菌属和链球菌属以及囊性纤维化病原体金黄色葡萄球菌和铜绿假单胞菌。铜绿假单胞菌和金黄色葡萄球菌的种群复制其染色体库的速度比健康气道微生物菌群中常见居民的种群要慢。在微生物宏基因组中评估生长动态可能成为诊断和管理囊性纤维化细菌感染的决策工具。
