Mosesso Niccolò, Bläske Tobias, Nagel Marie-Kristin, Laumann Michael, Isono Erika
Chair of Plant Physiology and Biochemistry, Department of Biology, University of Konstanz, Konstanz, Germany.
Electron Microscopy Centre, Department of Biology, University of Konstanz, Konstanz, Germany.
Front Plant Sci. 2019 Jan 9;9:1972. doi: 10.3389/fpls.2018.01972. eCollection 2018.
Clathrin coated vesicles (CCVs) mediate endocytosis of plasma membrane proteins and deliver their content to the endosomes for either subsequent recycling to the plasma membrane or transport to the vacuole for degradation. CCVs assemble also at the trans-Golgi network (TGN) and is responsible for the transport of proteins to other membranes. Oligomerization of clathrin and recruitment of adaptor protein complexes promote the budding and the release of CCVs. However, many of the details during plant CCV formation are not completely elucidated. The analysis of isolated CCVs is therefore important to better understand the formation of plant CCVs, their cargos and the regulation of clathrin-mediated transport processes. In this article, we describe an optimized method to isolate CCVs from seedlings.
网格蛋白包被囊泡（CCV）介导质膜蛋白的内吞作用，并将其内容物输送到内体，以便随后循环到质膜或运输到液泡进行降解。CCV也在反式高尔基体网络（TGN）处组装，并负责将蛋白质运输到其他膜。网格蛋白的寡聚化和衔接蛋白复合物的募集促进了CCV的出芽和释放。然而，植物CCV形成过程中的许多细节尚未完全阐明。因此，对分离的CCV进行分析对于更好地理解植物CCV的形成、其货物以及网格蛋白介导的运输过程的调节非常重要。在本文中，我们描述了一种从幼苗中分离CCV的优化方法。
