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不同来源视网膜前膜胶质样细胞中祖细胞标志物的表达

Expression of Progenitor Cell Markers in the Glial-Like Cells of Epiretinal Membranes of Different Origins.

作者信息

Yan Xiaohe, Andresen Pål, Lumi Xhevat, Chen Qingshan, Petrovski Goran

机构信息

Shenzhen Key Laboratory of Ophthalmology, Shenzhen Eye Hospital, Jinan University, Shenzhen 518040, China.

School of Optometry, Shenzhen University, Shenzhen 518040, China.

出版信息

J Ophthalmol. 2018 Dec 27;2018:7096326. doi: 10.1155/2018/7096326. eCollection 2018.

DOI:10.1155/2018/7096326
PMID:30687547
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6327511/
Abstract

PURPOSE

To investigate the expression of progenitor cell markers (Sox2, Nestin, and Pax2) in idiopathic epiretinal membranes (iERMs) and nonidiopathic epiretinal membranes (niERMs) in relation to glial cell marker expression.

METHODS

ERMs were obtained from patients with iERMs and niERMs of different origins: proliferative diabetic retinopathy (PDR), proliferative vitreoretinopathy (PVR), and uveitis. The membranes were studied by flat-mount or sectional immunohistochemistry for expression of progenitor cell markers as well as glial (GFAP) and proliferation (Ki-67) markers.

RESULTS

Cells in the ERMs express strong GFAP, with strong Pax2 expression in the cell nuclei. Some of the GFAP-positive glial cells in all epiretinal membrane types colocalized with Sox2, Pax2, and Nestin. NiERMs are much more cellular than iERMs. Glial cells are more densely packed in all analyzed niERMs, whereas glial cells with long branches are found in the internal limiting membrane parts and the iERMs, which appear to form a local network by their processes.

CONCLUSION

The GFAP-positive glial cells in ERMs are not pure glial cells, and some of them express progenitor cell markers, which indicate that these cells may have potential for self-renewal and differentiation into more glial or neuroglial type of cells.

摘要

目的

研究祖细胞标志物（Sox2、巢蛋白和Pax2）在特发性视网膜前膜（iERM）和非特发性视网膜前膜（niERM）中的表达，并探讨其与胶质细胞标志物表达的关系。

方法

从不同病因的iERM和niERM患者中获取视网膜前膜，病因包括增殖性糖尿病视网膜病变（PDR）、增殖性玻璃体视网膜病变（PVR）和葡萄膜炎。通过平铺或切片免疫组织化学方法研究这些视网膜前膜中祖细胞标志物以及胶质细胞（胶质纤维酸性蛋白，GFAP）和增殖标志物（Ki-67）的表达情况。

结果

视网膜前膜中的细胞GFAP表达强烈，细胞核中Pax2表达也强烈。在所有类型的视网膜前膜中，一些GFAP阳性胶质细胞与Sox2、Pax2和巢蛋白共定位。niERM比iERM细胞更多。在所有分析的niERM中，胶质细胞堆积更密集，而在内部限制膜部分和iERM中发现有长分支的胶质细胞，它们的突起似乎形成了一个局部网络。

结论

视网膜前膜中GFAP阳性胶质细胞并非纯粹的胶质细胞，其中一些表达祖细胞标志物，这表明这些细胞可能具有自我更新以及分化为更多胶质或神经胶质类型细胞的潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7c1/6327511/cf817f31e791/JOPH2018-7096326.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7c1/6327511/9168b1067644/JOPH2018-7096326.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7c1/6327511/872dcc287379/JOPH2018-7096326.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7c1/6327511/b697af1161c4/JOPH2018-7096326.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7c1/6327511/0b4a8ef7c483/JOPH2018-7096326.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7c1/6327511/cf817f31e791/JOPH2018-7096326.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7c1/6327511/9168b1067644/JOPH2018-7096326.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7c1/6327511/872dcc287379/JOPH2018-7096326.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7c1/6327511/b697af1161c4/JOPH2018-7096326.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7c1/6327511/0b4a8ef7c483/JOPH2018-7096326.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7c1/6327511/cf817f31e791/JOPH2018-7096326.005.jpg

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