Worthley C S, Byrne M J, Hickman R
Department of Surgery, University of Cape Town, Republic of South Africa.
Urol Res. 1988;16(6):449-54. doi: 10.1007/BF00280028.
Conventional creatinine clearance studies in the pig are complicated by difficulties with catheterisation, infection, accurate collection and active creatinine renal tubular reabsorption. We compared a single injection, 99mTc-DTPA elimination method with creatinine clearance. Nineteen pairs of GFR estimations were performed in 10 pigs: 7 in normal pigs and 12 following bile duct ligation and/or nephrectomy. Red cell and plasma protein binding of the isotope and its hepatobiliary excretion was assessed. Absolute and weight normalised endogenous creatinine clearances correlated significantly with 99mTc-DTPA elimination. 99mTc-DTPA red cell binding and hepatobiliary excretion were negligible. Thus, 99mTc-DTPA elimination is a valid indicator of changes in renal function in grouped porcine studies, particularly in the biliary obstruction model. However, isotope plasma protein binding was high in the 2 normal pigs assessed. Individual accuracy would be improved by routine protein binding correction, unless consistently low for a particular preparation.
猪的传统肌酐清除率研究因插管困难、感染、准确收集以及肌酐肾小管主动重吸收等问题而变得复杂。我们将单次注射99mTc-DTPA清除法与肌酐清除率进行了比较。对10头猪进行了19对肾小球滤过率（GFR）评估：7对在正常猪中进行，12对在胆管结扎和/或肾切除术后进行。评估了同位素的红细胞和血浆蛋白结合及其肝胆排泄情况。绝对和体重标准化的内生肌酐清除率与99mTc-DTPA清除率显著相关。99mTc-DTPA的红细胞结合和肝胆排泄可忽略不计。因此，在分组猪研究中，特别是在胆道梗阻模型中，99mTc-DTPA清除率是肾功能变化的有效指标。然而，在所评估的2头正常猪中，同位素血浆蛋白结合率较高。除非特定制剂的蛋白结合率一直较低，否则通过常规蛋白结合校正可提高个体准确性。
