Ten Leonid N, Jeon Na Yeong, Li Weilan, Kim Myung Kyum, Cho Young-Je, Jung Hee-Young
School of Applied Biosciences, Kyungpook National University, Daegu, 41566, Republic of Korea.
Department of Bio and Environmental Technology, Seoul Women's University, Seoul, 01797, Republic of Korea.
Antonie Van Leeuwenhoek. 2019 Jul;112(7):1019-1028. doi: 10.1007/s10482-019-01236-0. Epub 2019 Jan 28.
A bacterial strain, 1-3-3-3, was isolated from a soil sample collected in Jeollabuk-do province, South Korea. Cells were observed to be Gram-stain negative, short rod-shaped and colonies to be red-pink in colour. Analysis of 16S rRNA gene sequences identified this strain as a member of the genus Hymenobacter in the family Hymenobacteraceae, with high levels of 16S rRNA sequence similarity with Hymenobacter algoricola VUG-A23a (98.0%), Hymenobacter knuensis 16F7C-2 (97.9%), Hymenobacter fastidiosus VUG-A124 (97.1%), Hymenobacter elongatus VUG-A112 (97.0%), Hymenobacter chitinivorans Txc1 (97.0%) and Hymenobacter aquaticus 16F3P (96.7%). Growth of strain 1-3-3-3 was observed at 10-30 °C, pH 6-8 and in the presence of 0-1.0% NaCl. The genomic G + C content was determined to be 61.6 mol %. The predominant respiratory quinone of the isolate was found to be MK-7; the major fatty acids were identified as iso-C (19.9%), summed feature 3 (Cω7c/Cω6c, 19.7%), summed feature 4 (iso-C I/anteiso-C B, 17.8%), Cω5c (12.5%) and anteiso-C (11.2%), and the major polar lipid was found to be phosphatidylethanolamine. The phenotypic and chemotaxonomic data support the affiliation of strain 1-3-3-3 with the genus Hymenobacter. However, the DNA-DNA relatedness between the isolate and its closest phylogenetic neighbours was lower than 34%. The DNA-DNA hybridization result and the differentiating phenotypic properties clearly indicate that strain 1-3-3-3 represents a novel species in the genus Hymenobacter, for which the name Hymenobacter persicinus sp. nov. is proposed. The type strain is 1-3-3-3 (= KCTC 52742 = JCM 32191).
从韩国全罗北道采集的土壤样本中分离出一株细菌菌株1-3-3-3。观察到细胞革兰氏染色阴性，呈短杆状，菌落颜色为红粉色。16S rRNA基因序列分析确定该菌株为嗜冷杆菌科嗜冷杆菌属的成员，与Algoricola嗜冷杆菌VUG-A23a（98.0%）、庆熙嗜冷杆菌16F7C-2（97.9%）、苛求嗜冷杆菌VUG-A124（97.1%）、细长嗜冷杆菌VUG-A112（97.0%）、几丁质食嗜冷杆菌Txc1（97.0%）和水生嗜冷杆菌16F3P（96.7%）的16S rRNA序列相似度较高。观察到菌株1-3-3-3在10-30°C、pH 6-8以及0-1.0% NaCl存在的条件下生长。基因组G+C含量测定为61.6 mol%。发现该分离株的主要呼吸醌为MK-7；主要脂肪酸鉴定为异-C（19.9%）、总和特征3（Cω7c/Cω6c，19.7%）、总和特征4（异-C I/anteiso-C B，17.8%）、Cω5c（12.5%）和anteiso-C（11.2%），主要极性脂为磷脂酰乙醇胺。表型和化学分类学数据支持菌株1-3-3-3属于嗜冷杆菌属。然而，该分离株与其最接近的系统发育邻居之间的DNA-DNA相关性低于34%。DNA-DNA杂交结果和不同的表型特征清楚地表明菌株1-3-3-3代表嗜冷杆菌属中的一个新物种，为此提出名为Persicinus嗜冷杆菌的新物种名称。模式菌株为1-3-3-3（=KCTC 52742=JCM 32191）。
