Oğuzoğlu Tuba Çiğdem, Koç Bahattin Taylan, Coşkun Nüvit, Doğan Fırat, Duran-Yelken Selda
Department of Virology, Veterinary Faculty, Ankara University, 06110, Dışkapı-Ankara, Turkey.
Department of Virology, Veterinary Faculty, Adnan Menderes University, Aydın, Turkey.
Trop Anim Health Prod. 2019 Jun;51(5):1083-1087. doi: 10.1007/s11250-018-01787-w. Epub 2019 Jan 28.
As ubiquitous pathogens, bovine virus diarrhea viruses (BVDVs) in cattle have been reported several times in Turkey. Over time, the frequency and importance of this infection has increased for the livestock industries. A total of 1291 animals were sampled from a dairy herd in Turkey suspected of BVDV clinical signs, for instance, reproductive failures (abortion, congenital malformations in calves, repeat breeding, etc.) and interdigital phlegmon in adult animals. Reverse transcription polymerase chain reactions (RT-PCRs) were made by using targeted 5' untranslated region (UTR), N, E2, and NS2-3 pestiviral gene region primers for antigen ELISA-positive samples (n = 20). The obtained amplicons were sequenced. Sequence results showed the presence of a new subgroup in Pestivirus A species. This paper describes the nucleotide sequences of a new BVDV 1 (BVDV 1-v) subgroup member.
作为普遍存在的病原体，牛病毒性腹泻病毒（BVDVs）在土耳其的牛群中已被多次报道。随着时间的推移，这种感染对畜牧业的频率和重要性不断增加。从土耳其一个疑似感染牛病毒性腹泻病毒临床症状（如繁殖失败，包括流产、犊牛先天性畸形、反复配种等）的奶牛群中总共采集了1291份样本，同时也采集了成年动物的指间蜂窝织炎样本。针对抗原酶联免疫吸附测定（ELISA）呈阳性的样本（n = 20），使用靶向5'非翻译区（UTR）、N、E2和NS2-3瘟病毒基因区域引物进行逆转录聚合酶链反应（RT-PCR）。对获得的扩增子进行测序。序列结果显示瘟病毒A种中存在一个新的亚群。本文描述了一种新的牛病毒性腹泻病毒1型（BVDV 1-v）亚群成员的核苷酸序列。
