Sports Medicine Research and Testing Laboratory, University of Utah School of Medicine, Utah, USA.
Division of Physical medicine and Rehabilitation, University of Utah School of Medicine, Utah, USA.
Drug Test Anal. 2019 Jun;11(6):782-791. doi: 10.1002/dta.2571. Epub 2019 Feb 20.
The hydration status of an athlete at the time of a doping control sample collection is an important factor to consider when reviewing athlete biological passports (ABPs). Dehydration results in a reduction of the circulating plasma volume (PV), which may lead to artificially high values of some blood parameters. This study aimed to identify whether serum albumin could serve as a single marker of fluid shifts, which are not currently accounted for in the hematological passport. An additional marker could be used to assist experts when interpreting irregularities in the ABP.
Twelve subjects underwent multiple controlled exercise trials designed to induce varying levels of PV shifts. Pre-exercise blood samples were collected to establish baseline values for individual passports. During exercise interventions, blood samples were collected before the start of exercise and at 10 minute, 1 hour, 2 hours, and 24 hours following exercise.
Significant increases in hematological parameters - hemoglobin [Hb], hematocrit (HCT), albumin (ALB), and calculated OFF-score - were identified at varying time points following fluid shift-inducing exercise. Changes in ALB correlated strongly with changes in [Hb] (r = 0.753) and with estimated PV shifts (r = -0.764). In analyzing ABPs, the resulting increases in Hb did not trigger any atypical findings at 99% specificity.
Monitoring changes in ALB longitudinally may assist experts when reviewing PV shifts in the biological passport.
在审查运动员生物护照（ABP）时，考虑运动员在接受兴奋剂控制样本采集时的水合状态是一个重要因素。脱水会导致循环血浆体积（PV）减少，这可能导致一些血液参数人为升高。本研究旨在确定血清白蛋白是否可以作为一种单一标志物，来反映目前血液护照中未考虑到的液体转移。增加一个标志物可以帮助专家解释 ABP 中的不规则情况。
12 名受试者接受了多次受控运动试验，旨在诱导不同程度的 PV 转移。在运动干预之前采集血样，以建立个人护照的基线值。在运动过程中，分别在运动前、运动后 10 分钟、1 小时、2 小时和 24 小时采集血样。
在诱导液体转移的运动后不同时间点，血液学参数——血红蛋白（Hb）、红细胞压积（HCT）、白蛋白（ALB）和计算的 OFF 评分——显著增加。ALB 的变化与 Hb 的变化呈强相关（r=0.753），与估计的 PV 转移呈负相关（r=-0.764）。在分析 ABP 时，Hb 的增加在 99%的特异性下没有引发任何非典型发现。
纵向监测 ALB 的变化可能有助于专家在审查生物护照中的 PV 转移时提供帮助。
