Astolfi Tiffany, Schumacher Yorck Olaf, Crettaz von Roten Fabienne, Saugy Martial, Faiss Raphael
REDs, Research and Expertise in antiDoping sciences, University of Lausanne, Lausanne, Switzerland.
ISSUL, Institute of Sport Sciences, University of Lausanne, Lausanne, Switzerland.
Int J Lab Hematol. 2020 Feb;42(1):61-67. doi: 10.1111/ijlh.13140. Epub 2019 Dec 10.
The Athlete's Biological Passport (ABP) is a tool for the indirect detection of blood doping. Guidelines from the World Anti-Doping Agency (WADA) require a 2 hours delay after any physical exercise, and to be seated for 10 minutes before collecting an ABP sample. This study investigated posture-related hematological variations with changes in body position during blood sampling.
Ten successive venous blood samples from 38 subjects were collected in three situations: immediately after 10-minutes of normalized activity (B1), after 10-minutes seated (B2, typical reference sample in an anti-doping context), after a 50 m walk (B3), after 5 and 10-minutes in a seated position again (B4 and B5), and finally after 5-30 minutes supine (B6-B10). Hemoglobin concentration [Hb] and hematocrit (Hct) were determined by flow cytometry to assess putative posture-related variations.
Reticulocytes percentage was unchanged in all conditions, [Hb] and Hct were stable after at least 10-minutes in a seated position. Due to shifts in plasma volume, [Hb] and Hct increased slightly but significantly higher after changing posture for a short walk (+0.1 gr/dL [P = .008] and +0.4% [P = .01] respectively), but readjusted to previous levels after only 5 minutes. Supine position (>10 minutes) induced decreases of [Hb] (-0.2 g/dL in average, P < .01) and Hct (-1.1%, in average, P < .01).
The observed variations in [Hb] and Hct may have minor clinical significance, while they underline the need to follow strict guidelines for posture before and during blood sampling in an anti-doping context.
运动员生物护照（ABP）是一种间接检测血液兴奋剂的工具。世界反兴奋剂机构（WADA）的指南要求在任何体育锻炼后延迟2小时，并在采集ABP样本前静坐10分钟。本研究调查了采血过程中体位变化引起的与姿势相关的血液学变化。
在三种情况下从38名受试者中连续采集10份静脉血样本：正常活动10分钟后立即采集（B1）、静坐10分钟后采集（B2，反兴奋剂背景下的典型参考样本）、50米步行后采集（B3）、再次静坐5分钟和10分钟后采集（B4和B5），最后仰卧5 - 30分钟后采集（B6 - B10）。通过流式细胞术测定血红蛋白浓度[Hb]和血细胞比容（Hct），以评估假定的与姿势相关的变化。
在所有情况下网织红细胞百分比均无变化，静坐至少10分钟后[Hb]和Hct稳定。由于血浆体积的变化，短距离步行改变姿势后[Hb]和Hct略有增加但显著升高（分别为+0.1 g/dL [P = 0.008]和+0.4% [P = 0.01]），但仅5分钟后就重新调整到先前水平。仰卧位（>10分钟）导致[Hb]平均下降（-0.2 g/dL，P < 0.01）和Hct平均下降（-1.1%，P < 0.01）。
观察到的[Hb]和Hct变化可能具有较小的临床意义，但它们强调了在反兴奋剂背景下采血前和采血过程中遵循严格姿势指南的必要性。
