Department of Food Hygiene and Environmental Health, Faculty of Veterinary Medicine, University of Helsinki, Helsinki, Finland
Department of Food Hygiene and Environmental Health, Faculty of Veterinary Medicine, University of Helsinki, Helsinki, Finland.
J Bacteriol. 2019 Mar 26;201(8). doi: 10.1128/JB.00759-18. Print 2019 Apr 15.
and are the most common causes of bacterial gastroenteritis in the world. Ganglioside mimicry by lipooligosaccharide (LOS) is the triggering factor of Guillain-Barré syndrome (GBS), an acute polyneuropathy. Sialyltransferases from glycosyltransferase family 42 (GT-42) are essential for the expression of ganglioside mimics in Recently, two novel GT-42 genes, and , have been identified in Despite being present in ∼11% of currently available genomes, the biological role of and is unknown. In the present investigation, mutation studies with two strains expressing either or were performed and mass spectrometry was used to investigate differences in the chemical composition of LOS. Attempts were made to identify donor and acceptor molecules using activity tests with recombinant GT-42 enzymes. Here we show that CstIV and CstV are involved in LOS biosynthesis. In particular, is associated with LOS sialylation, while is linked to the addition of a diacetylated nonulosonic acid residue. Despite the fact that a major foodborne pathogen, its glycobiology has been largely neglected. The genetic makeup of the lipooligosaccharide biosynthesis locus was largely unknown until recently. harbors a large set of genes associated with lipooligosaccharide biosynthesis, including genes for several putative glycosyltransferases involved in the synthesis of sialylated lipooligosaccharide in In the present study, was found to express lipooligosaccharide structures containing sialic acid and other nonulosonate acids. These findings have a strong impact on our understanding of ecology, host-pathogen interaction, and pathogenesis.
和 是世界上引起细菌性肠胃炎的最常见原因。神经节苷脂模拟物由脂寡糖 (LOS) 引起，是格林-巴利综合征 (GBS) 的触发因素，这是一种急性多发性神经病。糖基转移酶家族 42 (GT-42) 的唾液酸转移酶对于 在 中表达神经节苷脂模拟物是必不可少的。最近，在 中发现了两个新型 GT-42 基因 和 。尽管在目前可用的 基因组中约有 11%存在 和 ，但其生物学作用尚不清楚。在本研究中，对表达 或 的两个菌株进行了突变研究，并使用质谱法研究了 LOS 化学组成的差异。使用重组 GT-42 酶的 活性试验尝试鉴定供体和受体分子。在这里，我们表明 CstIV 和 CstV 参与了 LOS 的生物合成。特别是 与 LOS 唾液酸化有关，而 与添加二乙酰化非乳糖酸残基有关。尽管 是一种主要的食源性病原体，但它的糖生物学在很大程度上被忽视了。直到最近， 脂寡糖生物合成基因座的遗传组成才在很大程度上被人们所了解。 拥有与脂寡糖生物合成相关的大量基因，包括参与 在 中合成唾液酸化脂寡糖的几个假定糖基转移酶的基因。在本研究中，发现 表达含有唾液酸和其他非乳糖酸的脂寡糖结构。这些发现对我们理解 生态学、宿主-病原体相互作用和发病机制具有重要影响。
