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一种GntR家族转录因子调控生物膜形成及多个糖转运蛋白基因的表达。

A GntR Family Transcription Factor in Regulates Biofilm Formation and Expression of Multiple Sugar Transporter Genes.

作者信息

Li Zongbo, Xiang Zhenting, Zeng Jumei, Li Yuqing, Li Jiyao

机构信息

State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.

Division of Infectious Diseases, Boston Children's Hospital, Harvard Medical School, Boston, MA, United States.

出版信息

Front Microbiol. 2019 Jan 14;9:3224. doi: 10.3389/fmicb.2018.03224. eCollection 2018.

DOI:10.3389/fmicb.2018.03224
PMID:30692967
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6340165/
Abstract

GntR family transcription factors have been implicated in the regulation of carbohydrate transport and metabolism in many bacteria. However, the function of this transcription factor family is poorly studied in , which is a commensal bacterium in the human oral cavity and a well-known cariogenic pathogen. One of the most important virulence traits of is its ability to transport and metabolize carbohydrates. In this study, we identified a GntR transcription factor in named StsR (Sugar Transporter Systems Regulator). The deletion of the gene in caused a decrease in both the formation of biofilm and the production of extracellular polysaccharides (EPS) at early stage. Global gene expression profiling revealed that the expression levels of 188 genes were changed in the mutant, which could be clustered with the sugar PTS and ABC transporters. Furthermore, StsR protein was purified and its conserved DNA binding motif was determined using electrophoretic mobility shift assays (EMSA) and DNase I footprinting assays. Collectively, the results of this research indicate that StsR is an important transcription factor in that regulates the expression of sugar transporter genes, production of EPS and formation of biofilm.

摘要

GntR家族转录因子已被证明在许多细菌的碳水化合物运输和代谢调控中发挥作用。然而，在口腔链球菌中，这个转录因子家族的功能鲜少被研究，口腔链球菌是人类口腔中的一种共生细菌，也是一种著名的致龋病原体。口腔链球菌最重要的毒力特性之一是其运输和代谢碳水化合物的能力。在本研究中，我们在口腔链球菌中鉴定出一种名为StsR（糖转运系统调节因子）的GntR转录因子。在口腔链球菌中缺失stsR基因会导致生物膜形成和早期胞外多糖（EPS）产生减少。全基因组基因表达谱分析显示，在stsR突变体中188个基因的表达水平发生了变化，这些基因可与糖磷酸转移酶系统（PTS）和ABC转运蛋白聚类。此外，纯化了StsR蛋白，并使用电泳迁移率变动分析（EMSA）和DNase I足迹分析确定了其保守的DNA结合基序。总的来说，本研究结果表明，StsR是口腔链球菌中一个重要的转录因子，它调节糖转运基因的表达、EPS的产生和生物膜的形成。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7076/6340165/ff1ff993dd31/fmicb-09-03224-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7076/6340165/7c35f5812664/fmicb-09-03224-g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7076/6340165/507583fb4b84/fmicb-09-03224-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7076/6340165/a7b5b4f4e44a/fmicb-09-03224-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7076/6340165/fd2071ed8c1f/fmicb-09-03224-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7076/6340165/ac08690a14c1/fmicb-09-03224-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7076/6340165/ff1ff993dd31/fmicb-09-03224-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7076/6340165/7c35f5812664/fmicb-09-03224-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7076/6340165/dc335fac80ef/fmicb-09-03224-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7076/6340165/784a72ae7330/fmicb-09-03224-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7076/6340165/84399f9bf9ef/fmicb-09-03224-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7076/6340165/7cbc52c5ae95/fmicb-09-03224-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7076/6340165/507583fb4b84/fmicb-09-03224-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7076/6340165/a7b5b4f4e44a/fmicb-09-03224-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7076/6340165/fd2071ed8c1f/fmicb-09-03224-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7076/6340165/ac08690a14c1/fmicb-09-03224-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7076/6340165/ff1ff993dd31/fmicb-09-03224-g010.jpg

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