A GntR Family Transcription Factor in Regulates Biofilm Formation and Expression of Multiple Sugar Transporter Genes.

GntR family transcription factors have been implicated in the regulation of carbohydrate transport and metabolism in many bacteria. However, the function of this transcription factor family is poorly studied in , which is a commensal bacterium in the human oral cavity and a well-known cariogenic pathogen. One of the most important virulence traits of is its ability to transport and metabolize carbohydrates. In this study, we identified a GntR transcription factor in named StsR (Sugar Transporter Systems Regulator). The deletion of the gene in caused a decrease in both the formation of biofilm and the production of extracellular polysaccharides (EPS) at early stage. Global gene expression profiling revealed that the expression levels of 188 genes were changed in the mutant, which could be clustered with the sugar PTS and ABC transporters. Furthermore, StsR protein was purified and its conserved DNA binding motif was determined using electrophoretic mobility shift assays (EMSA) and DNase I footprinting assays. Collectively, the results of this research indicate that StsR is an important transcription factor in that regulates the expression of sugar transporter genes, production of EPS and formation of biofilm.