Liang Quanhui, Jiang Xiaoyuan, Hu Lingfei, Yin Zhe, Gao Bo, Zhao Yuee, Yang Wenhui, Yang Huiying, Tong Yigang, Li Weixuan, Jiang Lingxiao, Zhou Dongsheng
Department of Laboratory Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, China.
Department of Clinical Laboratory, The First People's Hospital of Foshan, Foshan, China.
Front Microbiol. 2019 Jan 14;9:3318. doi: 10.3389/fmicb.2018.03318. eCollection 2018.
IncHI plasmids could be divided into five different subgroups IncHI1-5. In this study, the complete nucleotide sequences of seven - or -carrying IncHI5 plasmids from , , and were determined and compared in detail with all the other four available sequenced IncHI5 plasmids. These plasmids carried conserved IncHI5 backbones composed of and a -like gene (replication), (partition), and (conjugal transfer). Integration of a number of accessory modules, through horizontal gene transfer, at various sites of IncHI5 backbones resulted in various deletions of surrounding backbone regions and thus considerable diversification of IncHI5 backbones. Among the accessory modules were three kinds of resistance accessory modules, namely Tn and two antibiotic resistance islands designated ARI-A and ARI-B. These two islands, inserted at two different fixed sites (one island was at one site and the other was at a different site) of IncHI5 backbones, were derived from the prototype Tn-family transposons Tn and Tn, respectively, and could be further discriminated as various intact transposons and transposon-like structures. The ARI-A or ARI-B islands from different IncHI5 plasmids carried distinct profiles of antimicrobial resistance markers and associated mobile elements, and complex events of transposition and homologous recombination accounted for assembly of these islands. The carbapenemase genes , and were identified within various class 1 integrons from ARI-A or ARI-B of the seven plasmids sequenced in this study. Data presented here would provide a deeper insight into diversification and evolution history of IncHI5 plasmids.
IncHI质粒可分为五个不同的亚组IncHI1 - 5。在本研究中，测定了来自[具体来源未给出]的七个携带[具体相关基因未明确给出]的IncHI5质粒的完整核苷酸序列，并与其他四个已测序的IncHI5质粒进行了详细比较。这些质粒携带由[相关基因未明确给出]和一个类[具体基因未明确给出]基因（复制）、[相关基因未明确给出]（分配）以及[相关基因未明确给出]（接合转移）组成的保守IncHI5骨架。通过水平基因转移，多个辅助模块整合到IncHI5骨架的不同位点，导致周围骨架区域的各种缺失，从而使IncHI5骨架产生了相当大的多样性。辅助模块中有三种抗性辅助模块，即Tn以及两个分别命名为ARI - A和ARI - B的抗生素抗性岛。这两个岛分别插入到IncHI5骨架的两个不同固定位点（一个岛在一个位点，另一个在不同位点），分别源自原型Tn家族转座子Tn和Tn，并且可以进一步区分为各种完整的转座子和转座子样结构。来自不同IncHI5质粒的ARI - A或ARI - B岛携带不同的抗菌抗性标记和相关移动元件谱，转座和同源重组的复杂事件解释了这些岛的组装。在本研究测序的七个质粒的ARI - A或ARI - B的各种1类整合子中鉴定出碳青霉烯酶基因[具体基因未明确给出]、[具体基因未明确给出]和[具体基因未明确给出]。此处呈现的数据将为深入了解IncHI5质粒的多样性和进化历史提供帮助。
