Luo Xinhua, Zhang Jin, Yuan Min, Mou Sihua, Xu Mengqiao, Hu Dakang, Ma Qinfei, Sun Lingfen, Li Piaopiao, Song Zhiwei, Yu Lianhua, Mu Kai
Department of Clinical Laboratory Medicine, Taizhou Municipal Hospital Affiliated with Taizhou University, Taizhou, 318000, People's Republic of China.
State Key Laboratory for Infectious Diseases Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Disease, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, People's Republic of China.
Infect Drug Resist. 2022 Apr 13;15:1831-1843. doi: 10.2147/IDR.S358839. eCollection 2022.
This study aimed to explore the genomic characterization of multidrug-resistant IncHI5-carrying strains and detailed genomic dissection of the IncHI5 plasmids.
Through whole-genome sequencing, the IncHI5 plasmid pK92-qnrS was obtained from a single clinical isolate K92. All complete genomes of strains from the Genome database of NCBI were collected and used to construct a maximum likelihood (ML) phylogenetic tree. The epidemiology and geographic distribution of all the strains were conducted. An extensive comparison of the seven IncHI5 plasmids of (one from this study, six from GenBank) was applied.
This study revealed that all strains carrying IncHI5 plasmids from different clonal groups were located in the southeast coastal area of China. The backbone regions of IncHI5 plasmids were composed of replicon ( and ), partition (), and conjugal transfer (/). The main accessory resistant regions of IncHI5 could be divided into two categories, Tn-related region and Tn-related region. These seven IncHI5 plasmids carried multiple drug-resistance genes which were all mediated by the mobile genetic elements (MGEs).
Data presented here help to provide an overall in-depth understanding of epidemiology and geographic distribution of IncHI5-carrying and the structure and evolutionary history of IncHI5 plasmids.
本研究旨在探索携带多重耐药IncHI5菌株的基因组特征，并对IncHI5质粒进行详细的基因组剖析。
通过全基因组测序，从单个临床分离株K92中获得IncHI5质粒pK92-qnrS。收集NCBI基因组数据库中所有菌株的完整基因组，用于构建最大似然（ML）系统发育树。对所有菌株进行了流行病学和地理分布研究。对7个IncHI5质粒（本研究中的1个，GenBank中的6个）进行了广泛比较。
本研究表明，所有携带来自不同克隆群的IncHI5质粒的菌株均位于中国东南沿海地区。IncHI5质粒的主干区域由复制子（和）、分区（）和接合转移（/）组成。IncHI5的主要辅助耐药区域可分为两类，Tn相关区域和Tn相关区域。这7个IncHI5质粒携带多个耐药基因，均由移动遗传元件（MGEs）介导。
本文提供的数据有助于全面深入了解携带IncHI5的菌株的流行病学和地理分布以及IncHI5质粒的结构和进化历史。
