山核桃对人表皮角质形成细胞中紫外线 B 辐射诱导的炎症反应的保护作用。
Protective effect of Juglans regia L. against ultraviolet B radiation induced inflammatory responses in human epidermal keratinocytes.
机构信息
Department of Zoology, Faculty of Science, Annamalai University, Annamalai Nagar 608 002, Tamilnadu, India.
Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalai Nagar 608 002, Tamilnadu, India.
出版信息
Phytomedicine. 2018 Mar 15;42:100-111. doi: 10.1016/j.phymed.2018.03.024. Epub 2018 Mar 13.
BACKGROUND
Juglans regia L. has a history of traditional medicinal use for the treatment of various maladies and have been documented with significant antioxidant and antiinflammatory properties. Although all parts of the plant are medicinally important, but male the flower of the plant has not been yet investigated against the photo-damage.
PURPOSE
The present study, we sought to determine the photoprotective effect of the male flower of J. regia L. against ultraviolet-B radiation-induced inflammatory responses in human skin cells.
METHODS
The profile of pharmacological active compounds present in the male flower of J. regia was analyzed by GC-MS. Then, the antioxidant property of methanolic extract of J. regia (MEJR) was analyzed by in vitro free radical scavenging assays. Further, we analyzed the sun protection factor of this extract by spectrophotometry. Moreover, we investigated the photoprotective effect of MEJR against UVB induced inflammatory signaling in human epidermal cells. Human skin epidermal keratinocytes (HaCaT) were pretreated with the MEJR (80 µg/ml), 30 min prior to UVB-irradiation at a dose of 20 mJ/cm and were investigated for lipid peroxidation, enzymatic antioxidants activity, apoptosis and inflammatory markers expression level.
RESULTS
The GC-MS results showed the presence of good amount of pharmacologically active compounds in the MEJR. We observed that the MEJR possess significant free radical scavenging activity and it was comparable with standard antioxidants. Further, the MEJR exhibits 8.8 sun-protection-factor (SPF) value. Pretreatment with MEJR, 30 min prior to UVB-irradiation, prevented ROS generation, lipid peroxidation and restored the activity of antioxidant status in HaCaT cells. Moreover, MEJR pretreatment significantly prevented UVB activated inflammatory markers like TNF-α, IL-1, IL-6, NF-κB, COX-2 in HaCaT.
CONCLUSION
The present findings suggest that MEJR exhibit photoprotective effects and hence it may be useful for the treatment of inflammation related responses. The pharmacological mechanism of MEJR partly associated with its UV absorbance, modulation of inflammatory signaling as well as due to its free radical scavenging capability.
背景
胡桃 J. regia L. 具有治疗各种疾病的传统药用历史，具有显著的抗氧化和抗炎特性。虽然植物的所有部分都具有药用价值，但男性植物的花尚未针对光损伤进行研究。
目的
本研究中，我们试图确定胡桃雄花对人皮肤细胞紫外线-B 辐射诱导的炎症反应的光保护作用。
方法
通过 GC-MS 分析胡桃雄花中存在的药理活性化合物的特征。然后，通过体外自由基清除试验分析胡桃甲醇提取物（MEJR）的抗氧化性能。此外，我们通过分光光度法分析了该提取物的防晒系数。此外，我们研究了 MEJR 对人表皮细胞中 UVB 诱导的炎症信号的光保护作用。人皮肤表皮角质形成细胞（HaCaT）用 MEJR（80μg/ml）预处理，30 分钟后用 20mJ/cm 的剂量进行 UVB 照射，并研究脂质过氧化、酶抗氧化剂活性、凋亡和炎症标志物表达水平。
结果
GC-MS 结果表明 MEJR 中存在大量具有药理活性的化合物。我们观察到 MEJR 具有显著的自由基清除活性，与标准抗氧化剂相当。此外，MEJR 表现出 8.8 的防晒系数（SPF）值。在 UVB 照射前 30 分钟用 MEJR 预处理可防止 ROS 生成、脂质过氧化，并恢复 HaCaT 细胞的抗氧化状态活性。此外，MEJR 预处理可显著防止 TNF-α、IL-1、IL-6、NF-κB、COX-2 等炎症标志物在 HaCaT 中的激活。
结论
本研究结果表明，MEJR 具有光保护作用，因此可用于治疗炎症相关反应。MEJR 的药理机制部分与其对紫外线的吸收、炎症信号的调节以及其自由基清除能力有关。
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