Sun Yanling, Zou Jingwei, Sun Huigai, Song Wei, Wang Xiaoming, Li Hongjie
The National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI), Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081.
The National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI), Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081; and College of Life Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao 066004, China.
Plant Dis. 2015 Aug;99(8):1118-1124. doi: 10.1094/PDIS-10-14-1079-RE. Epub 2015 Jun 11.
Wheat powdery mildew (caused by Blumeria graminis f. sp. tritici) can be effectively managed by growing resistant cultivars. 'Liangxing 66' and 'Wennong 14' are the current winter wheat cultivars grown in northern China where powdery mildew is epidemic. Both cultivars have been demonstrated to carry single dominant genes for resistance to powdery mildew, tentatively designated PmLX66 and PmW14, on chromosome 5DS and share common linked markers with Pm2. Allelism tests were performed using a total of 15,657 plants of F segregating populations to determine the relationship between PmLX66, PmW14, and Pm2. All progeny from the crosses Liangxing 66 × 'Ulka/8Chancellor' (Ulka/8Cc), Wennong 14 × Ulka/8Cc, and Liangxing 66 × Wennong 14 were resistant when tested with B. graminis f. sp. tritici isolate E20, indicating that PmLX66 and PmW14 are allelic to Pm2 and to each other. Liangxing 66 was resistant to 76.7% of the 60 B. graminis f. sp. tritici isolates from northern China, a slightly smaller proportion than Ulka/8Cc (78.3%). However, Wennong 14 (85.0%) was more resistant against this set of B. graminis f. sp. tritici isolates than Ulka/8Cc and Liangxing 66. Liangxing 66 and Wennong 14 differed from Ulka/8Cc in respect to a number of B. graminis f. sp. tritici isolates. Based on these findings, PmLX66 and PmW14 are new alleles at the Pm2 locus.
小麦白粉病（由小麦白粉菌引起）可通过种植抗病品种得到有效控制。“良星66”和“温农14”是中国北方白粉病流行地区目前种植的冬小麦品种。这两个品种均已证明在5DS染色体上携带抗白粉病的单个显性基因，暂命名为PmLX66和PmW14，并与Pm2共享共同的连锁标记。利用F分离群体的总共15657株植株进行等位性测试，以确定PmLX66、PmW14和Pm2之间的关系。当用小麦白粉菌小种E20进行测试时，“良星66”ד乌尔卡/8总理”（乌尔卡/8Cc）、“温农14”×乌尔卡/8Cc以及“良星66”ד温农14”杂交组合的所有后代均表现抗病，这表明PmLX66和PmW14与Pm2等位且彼此等位。“良星66”对来自中国北方的60个小麦白粉菌小种分离株中的76.7%具有抗性，比例略低于乌尔卡/8Cc（78.3%）。然而，“温农14”（85.0%）对这组小麦白粉菌小种分离株的抗性比乌尔卡/8Cc和“良星66”更强。“良星66”和“温农14”在一些小麦白粉菌小种分离株方面与乌尔卡/8Cc不同。基于这些发现，PmLX66和PmW14是Pm2位点的新等位基因。
