Jia Mengshu, Xu Hongxing, Liu Cheng, Mao Ruixi, Li Haosheng, Liu Jianjun, Du Wenxiao, Wang Wenrui, Zhang Xu, Han Ran, Wang Xiaolu, Wu Liru, Liang Xiao, Song Jiancheng, He Huagang, Ma Pengtao
School of Life Sciences, Yantai University, Yantai, China.
State Key Laboratory of Crop Stress Adaptation and Improvement, School of Life Sciences, Henan University, Kaifeng, China.
Front Genet. 2020 Apr 2;11:241. doi: 10.3389/fgene.2020.00241. eCollection 2020.
Powdery mildew infection of wheat ( L.), caused by f. sp. (), is a destructive disease that threatens yield and quality worldwide. The most effective and preferred means for the control of the disease is to identify broad-spectrum resistance genes for breeding, especially the genes derived from elite cultivars that exhibit desirable agronomic traits. Jimai 23 is a Chinese wheat cultivar with superior agronomic performance, high-quality characteristics, and effective resistance to powdery mildew at all growth stages. Genetic analysis indicated that powdery mildew resistance in Jimai 23 was mediated by a single dominant gene, tentatively designated . Using bulked segregant RNA-Seq (BSR-Seq), a series of markers was developed and used to map was then located at the locus on the short arm of chromosome 5D (5DS). Resistance spectrum analysis demonstrated that provided a broad resistance spectrum different from that of the documented alleles, indicating that is most likely a new allele of . In view of these combined agronomic, quality, and resistance findings, is expected to be a valuable resistance gene in wheat breeding. To efficiently use in breeding, the closely linked markers of were evaluated and confirmed to be applicable for marker-assisted selection (MAS). Using these markers, a series of resistant breeding lines with high resistance and desirable agronomic performance was selected from the crosses involving , resulting in improved powdery mildew resistance of these lines.
由小麦白粉菌（Blumeria graminis f. sp. tritici）引起的小麦白粉病是一种全球性的毁灭性病害，威胁着小麦的产量和品质。控制该病害最有效且首选的方法是鉴定用于育种的广谱抗性基因，尤其是来自具有优良农艺性状的优良品种的基因。济麦23是一个中国小麦品种，具有优异的农艺性能、优质特性，且在各个生育阶段对白粉病均具有有效的抗性。遗传分析表明，济麦23对白粉病的抗性由一个单显性基因介导，暂命名为PmJ23。利用混合分离群体RNA测序（BSR-Seq）开发了一系列标记，并用于定位PmJ23，结果表明它位于5D染色体短臂（5DS）上的Pm2位点。抗性谱分析表明，PmJ23提供了与已报道的Pm2等位基因不同的广谱抗性，这表明PmJ23很可能是Pm2的一个新等位基因。鉴于这些综合的农艺、品质和抗性研究结果，PmJ23有望成为小麦育种中一个有价值的抗性基因。为了在育种中有效利用PmJ23，对其紧密连锁标记进行了评估并证实适用于标记辅助选择（MAS）。利用这些标记，从涉及PmJ23的杂交组合中筛选出了一系列具有高抗性和优良农艺性能的抗性育种系，从而提高了这些品系对白粉病的抗性。
