Biotechnology, guangdong polytechnic science and trade, Guangzhou 510640, Guangdong, China.
School of Medical Technology and Nursing, Shenzhen Polytechnic, Shenzhen 518000, Guangdong, China.
Biomed Environ Sci. 2019 Jan;32(1):11-21. doi: 10.3967/bes2019.002.
A strain of Aspergillus niger (A. niger), capable of releasing bound phenolic acids from wheat bran, was isolated. This strain was identified by gene sequence identification. The antioxidant and anti-inflammatory capacity of ferulic acid released from wheat bran by this A. niger strain (FA-WB) were evaluated.
Molecular identification techniques based on PCR analysis of specific genomic sequences were conducted; antioxidant ability was examined using oxygen radical absorbance capacity (ORAC), cellular antioxidant activity (CAA) assays, and erythrocyte hemolysis assays. RAW264.7 cells were used as a model to detect anti-inflammatory activity.
The filamentous fungal isolate was identified to be A. niger. ORAC and CAA assay showed that FA-WB had better antioxidant activity than that of the ferulic acid standard. The erythrocyte hemolysis assay results suggested that FA-WB could attenuate AAPH-induced oxidative stress through inhibition of reactive oxy gen species (ROS) generation. FA-WB could significantly restore the AAPH-induced increase in intracellular antioxidant enzyme activities to normal levels as well as inhibit the intracellular malondialdehyde formation. TNF-a, IL-6, and NO levels indicated that FA-WB can inhibit the inflammation induced by lipopolysaccharide (LPS).
Ferulic acid released from wheat bran by a new strain of A. niger had good anti-inflammatory activity and better antioxidant ability than standard ferulic acid.
分离出一株能够从麦麸中释放结合型酚酸的黑曲霉（Aspergillus niger）菌株。通过基因序列鉴定对该菌株进行鉴定。评估该黑曲霉菌株（FA-WB）从麦麸中释放的阿魏酸（ferulic acid）的抗氧化和抗炎能力。
采用基于特定基因组序列 PCR 分析的分子鉴定技术；通过氧自由基吸收能力（ORAC）、细胞抗氧化活性（CAA）测定和红细胞溶血测定来检测抗氧化能力。使用 RAW264.7 细胞作为模型来检测抗炎活性。
丝状真菌分离物被鉴定为黑曲霉。ORAC 和 CAA 测定表明，FA-WB 的抗氧化活性优于阿魏酸标准品。红细胞溶血试验结果表明，FA-WB 可通过抑制活性氧（ROS）的产生来减轻 AAPH 诱导的氧化应激。FA-WB 可显著将 AAPH 诱导的细胞内抗氧化酶活性升高恢复至正常水平，并抑制细胞内丙二醛的形成。TNF-α、IL-6 和 NO 水平表明，FA-WB 可抑制脂多糖（LPS）诱导的炎症。
一株新型黑曲霉从麦麸中释放的阿魏酸具有良好的抗炎活性和比标准阿魏酸更好的抗氧化能力。
