四种天然产物的抗氧化潜力及通过抑制人多发性骨髓瘤细胞系 U266 活力的抗癌治疗策略。

Antioxidant Potential of Four Species of Natural Product and Therapeutic Strategies for Cancer through Suppression of Viability in the Human Multiple Myeloma Cell Line U266.

机构信息

Laboratory of Biochemistry and Molecular Biology, Faculty of Sciences of Bizerte, University of Carthage, 1054, Tunisia.

Research Unit of Macromolecular Biochemistry and Genetics, Faculty of Sciences of Gfasa, 2112, Tunisia.

出版信息

Biomed Environ Sci. 2019 Jan;32(1):22-33. doi: 10.3967/bes2019.003.

DOI:10.3967/bes2019.003

PMID:30696536

Abstract

OBJECTIVE

This research aimed to evaluate the protective effects of bioactive compounds such as phenolic acids, flavonoids, and tannins present in four species extracted with methanol.

METHODS

The total phenolic content of the methanolic extracts was measured spectrophotometrically. The effect of the extracts on cell viability in U266 cells was measured. The effects of extracts on free radical scavenging were assessed by the DPPH test and FRAP assay. Antibacterial effects of the natural products in this report were investigated by using the disc diffusion method.

RESULTS

Our results clearly demonstrated that the methanolic extracts were characterized by a high amount of phenolic compounds. It has been speculated that ME-TA and ME-TAl exhibit a significant (P < 0.05) and dose-dependent antiradical potential. The exposure of cells to high doses of extracts almost completely suppressed cell growth in vitro. ME-TA and ME-TAl showed significant cytotoxic effects at a concentration of 100 μg/mL in the U266 cell line. ME-TAl and ME-CF inhibited the growth of B. subtilis and S. aureus, respectively, to the same extent as 10 μg/μL of chloramphenicol at a concentration of 1 mg/mL.

CONCLUSION

Overall, these results suggest that plants used in traditional medicine have a novel application as free radical scavengers, bacterial inhibitors and tumor suppressors.

摘要

目的

本研究旨在评估甲醇提取的四种植物中所含的生物活性化合物（如酚酸、类黄酮和单宁）的保护作用。

方法

采用分光光度法测定甲醇提取物的总酚含量。测定提取物对 U266 细胞活力的影响。通过 DPPH 试验和 FRAP 测定法评估提取物的自由基清除作用。采用圆盘扩散法研究天然产物的抑菌作用。

结果

我们的结果清楚地表明，甲醇提取物的特点是含有大量的酚类化合物。据推测，ME-TA 和 ME-TAl 表现出显著的（P<0.05）和剂量依赖性的抗自由基潜力。暴露于高剂量提取物的细胞在体外几乎完全抑制了细胞生长。ME-TA 和 ME-TAl 在 U266 细胞系中浓度为 100μg/mL 时表现出显著的细胞毒性作用。ME-TAl 和 ME-CF 分别对枯草芽孢杆菌和金黄色葡萄球菌的生长表现出与 1mg/mL 浓度下 10μg/μL 的氯霉素相同的抑制作用。